23 Crassula lycopodioides溢泌物の化学組成とジャスモン酸メチルエステルによる制御

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Methyl jasmonate promoted leaf abscission of Crassula lycopodioides and the exudation from stems and leaves after leaf abscission when it was sprayed in whole plants. Major component of the exudate was identified as sucrose based on the results described below. (1) The reaction of the exudate was not positive in Somogyi-Nelson method but in phenol-sulfuric acid method. (2) The exudate was hydrolyzed with invertase of Candida utilis, after that showing the positive reaction in Somogyi-Nelson method. (3) Only glucose and mannose were identified in the exudate after hydrolysis with trifluoroacetic acid using a gas-liquid chromatograph (GLC). (4) Finally, a single peak corresponding to the retention time of sucrose was observed using a high performance liquid chromatograph (HPLC). Moreover, small amounts of potassium and sodium ions were found in the exudate. These results suggest that the exudate is phloem sap of Grassula Lycopodioides, and methyl jasmonate inhibits the synthesis of callose in sieve tubes of this plant.
1999-10-01