Aerogenous Spread of Primary Lung Adenocarcinoma Induces Ultrastructural Remodeling of the Alveolar Capillary Endothelium
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概要
- 論文の詳細を見る
To determine whether pulmonary alveolar capillaries manifest ultrastructural remodeling at areas of neoplastic invasion of primary lung adenocarcinomas, we examined 17 well-differentiated adenocarcinomas of lung (2 bronchioloalveolar and 15 papillary adenocarcinomas) by electron microscopy. The expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) was demonstrated by immunohistochemical stainings. VEGF messenger RNA (mRNA) isoforms were detected by reverse-transcription polymerase chain reaction (RT-PCR) in alveolar walls microdissected from normal and tumor-associated tissues. Cytoplasm of neoplastic cells expressed VEGF protein in all patients. Endothelial cell nuclei of alveolar capillaries showed positive reaction for PCNA. Alveolar capillary lumina were distended like venules, and some intercellular junctions remained open. The cytoplasm of the capillary endothelial cells was enlarged and developed numerous organelles such as Weibel-Palade bodies and vesiculovacuolar organelles, in contrast to marked attenuation in their normal counterpart. Capillary sprouting occurred from proper alveolar capillaries in 2 patients. Cytoplasmic segments became extremely attenuated and developed diaphragm-like fenestrae in 65% of the patients. A relatively higher expression of diffusable isoforms of VEGF mRNA was seen in the tumor-bearing alveolar walls than in normal walls. Expression of KDR (one of the VEGF receptors) mRNA in tumor exceeded that in normal tissues. These results suggest that diffusable isoforms of VEGF mRNA released from the neoplastic cells are deeply involved in the induction of growth activity of alveolar capillary endothelial cells as much as in the characterization of tumor-associated microvessels in primary lung adenocarcinomas.
- 日本医科大学の論文
- 2002-03-25
著者
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Takemura Tamiko
Pathology Laboratory Nippon Red Cross Hospital Center
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Takemura Tamiko
Department Of Pathology Japanese Red Cross Medical Center Tokyo
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Ghazizadeh M
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
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Shimizu Hajime
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
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JIN Enjing
Department of Pathology, Institute of Gerontology, Nippon Medical School
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KAWANAMI Oichi
Department of Pathology, Institute of Gerontology, Nippon Medical School
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Takemura Tamiko
Department Of Pathology Japanese Red Cross Medical Center
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OHAKI Yoshiharu
Department of Pathology, Nippon Medical School, Chiba Hokusou Hospital
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Ghazizadeh Mohammad
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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FUJIWARA MASAKAZU
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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NAGASHIMA MIKIO
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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PAN XIN
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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ARAI SATORU
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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GOMIBUCHI MAKOTO
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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Arai Satoru
Pathology Section, Hokusoh Hospital Nippon Medical School
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Gomibuchi Makoto
Surgery Section, Hokusoh Hospital Nippon Medical School
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Fujiwara Masakazu
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
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Ghazizadeh Mohammad
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
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Ohaki Yoshiharu
Pathology Section Hokusoh Hospital Nippon Medical School
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Ohaki Yoshiharu
Department Of Pathology Chiba Hokuso Hospital Nippon Medical School
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Pan Xin
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School:surgical Pathology
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Pan Xin
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
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Jin Enjing
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
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Jin Enjing
Department Of Pathology Institute Of Gerontology Nippon Medical School
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Arai Satoru
Pathology Section Hokusoh Hospital Nippon Medical School
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Arai Satoru
Department Of Dermatology Kitasato University School Of Medicine
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Nakajima Michiko
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
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Kawanami Oichi
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
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Kawanami Oichi
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
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Gomibuchi Makoto
Surgery Section Hokusoh Hospital Nippon Medical School
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Takemura Tamiko
Department Of Pathology Japan Red Cross Medical Center
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Shimizu Hajime
Department of Electronics and Computer Science, Tokyo University of Science
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