Angiogenesis and phenotypic alteration of alveolar capillary endothelium in areas of neoplastic cell spread in primary lung adenocarcinoma
スポンサーリンク
概要
- 論文の詳細を見る
Normal alveolar capillary endothelium is quiescent in nature and displays anticoagulant thrombomodulin (TM) on its surface. The cytoplasms of these endothelial cells are ultrastructurally non-fenestrated type, and they barely express von Willebrand factor (vWf). Alveolar fibrosis is accompanied by a capillary endothelium reactive for vWf, and a loss of TM expression. In primary lung adenocarcinoma, neovascularization occurs in association with alveolar fibrosis. In order to study basic factors related to angiogenesis and phenotypic changes of the capillaries located in tumorbearing alveolar walls, we examined 37 primary lung adenocarcinomas with electron microscopy and confocal laser scanning microscopy with antibodies for TM, vWf, vascular endothelial growth factor (VEGF), and its receptors (KDR and Flt-1), and proliferating markers (Ki-67/proliferating cell nuclear antigen). Tissues microdissected specifically from alveolar walls were used for reverse transcription-polymerase chain reaction (RT-PCR) to assess expressions of mRNA isoforms of VEGF and its receptors. New capillary branching was found by ultrastructural study in the alveolar walls in 12% of the patients. Nuclei of the capillary endothelial cells were reactive for proliferating cell markers. Endothelial fenestrae were developed in 65% of the patients, TM reactivity was lost in the alveolar capillaries, and their cell cytoplasms obtained a reactivity for vWf through a transitional mosaic-like distribution pattern of both antigens. Besides cytoplasmic VEGF expression in neoplastic cells, tumor-bearing alveolar walls showed significant expression of mRNA of VEGF_<165> and KDR. These findings imply that angiogenesis and phenotypic changes of the alveolar capillaries are closely related to a higher expression of tumor-associated VEGF_<165> and of KDR in the alveolar walls in primary lung adenocarcinoma.
- 日本医科大学の論文
- 2002-03-25
著者
-
Takemura Tamiko
Pathology Laboratory Nippon Red Cross Hospital Center
-
Ghazizadeh M
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
-
Shimizu Hajime
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
-
JIN Enjing
Department of Pathology, Institute of Gerontology, Nippon Medical School
-
KAWANAMI Oichi
Department of Pathology, Institute of Gerontology, Nippon Medical School
-
Ghazizadeh Mohammad
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
-
FUJIWARA MASAKAZU
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
-
NAGASHIMA MIKIO
Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
-
Ohaki Yoshiharu
Pathology Section, Hokusoh Hospital Nippon Medical School
-
Arai Satoru
Pathology Section, Hokusoh Hospital Nippon Medical School
-
Gomibuchi Makoto
Surgery Section, Hokusoh Hospital Nippon Medical School
-
Fujiwara Masakazu
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
-
Ghazizadeh Mohammad
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
-
Ohaki Yoshiharu
Pathology Section Hokusoh Hospital Nippon Medical School
-
Jin Enjing
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
-
Jin Enjing
Department Of Pathology Institute Of Gerontology Nippon Medical School
-
Arai Satoru
Pathology Section Hokusoh Hospital Nippon Medical School
-
Nakajima Michiko
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
-
Kawanami Oichi
Departments Of Molecular Pathology And Biochemistry And Cell Biology Institute Of Development And Ag
-
Kawanami Oichi
Department Of Molecular Pathology Institute Of Gerontology Nippon Medical School
-
Gomibuchi Makoto
Surgery Section Hokusoh Hospital Nippon Medical School
-
Shimizu Hajime
Department of Electronics and Computer Science, Tokyo University of Science
関連論文
- Amplification, up-regulation and over-expression of C3G (CRK SH3 domain-binding guanine nucleotide-releasing factor) in non-small cell lung cancers
- Amplification, up-regulation and over-expression of DVL-1, the human counterpart of the Drosophila disheveled gene, in primary breast cancers
- Magnetic Properties of Superconducting and Nonsuperconducting GdBa_2(Cu_M_x)_3O_7 (M=Fe,Co,Ni:x=0-0.19)
- DNA alterations during multi-step development of human hepatocellular carcinomas revealed by laser capture microdissection
- Hypermethylation associated with inactivation of the SOCS-1 gene, a JAK/STAT inhibitor, in human hepatoblastomas
- Ultrastructural Characteristics of Myocardial and Coronary Microvascular Lesions in Kawasaki Disease
- Histological study of papillary thyroid carcinoma treated with percutaneous ethanol injection therapy
- Aerogenous Spread of Primary Lung Adenocarcinoma Induces Ultrastructural Remodeling of the Alveolar Capillary Endothelium
- Angiogenesis and phenotypic alteration of alveolar capillary endothelium in areas of neoplastic cell spread in primary lung adenocarcinoma
- Angiogenesis and phenotypic alteration of alveolar capillary endothelium in areas of neoplastic cell spread in primary lung adenocarcinoma
- Mosaic-like distribution of endothelial cell antigens in capillaries and juxta-alveolar microvessels in the normal human lung
- Heterogeneous Distribution of Thrombomodulin and von Willebrand Factor in Endothelial Cells in the Human Pulmonary Microvessels
- Mosaic-like distribution of endothelial cell antigens in capillaries and juxta-alveolar microvessels in the normal human lung
- Theoretical Investigation of Magnetic Structure and Hyperfine Field of NaCl-Type FeN
- Electronic,Structural and Magnetic Properties of Transition-Metal Mononitrides
- Immunohistochemical and ultrastructural studies of basal cells, Clara cells and bronchiolar cuboidal cells in normal human airways
- Immunohistochemical and ultrastructural studies of basal cells, Clara cells and bronchiolar cuboidal cells in normal human airways
- Multiple Magnetic Transitions in RuSr_2R_Ce_Cu_2O_(R = Gd, Eu) from Magnetization and ESR Spectroscopy(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Multiple Magnetic Transitions in RuSr_2R_Ce_Cu_2O_ (R = Gd, Eu) from Magnetization and ESR Spectroscopy
- High-Pressure FT-IR Spectra of Liquid and Crystalline CH_2F_2 up to 13 GPa
- Mode-Gruneisen Parameters of Molecular Crystals and Their Pressure Dependence : Some Halogenated Methanes
- High-Pressure Raman Study of Liquid and Crystalline CH_2F_2 up to 29 GPa
- Molecular Cytogenetic Characterization of Drug-resistant Leukemia Cell Lines by Comparative Genomic Hybridization and Fluorescence in situ Hybridization
- P1-13 Application of Citraconic Anhydride (CCA) for Antigen Retrieval Immunohistochemistry
- Anti-apoptotic PTD-FNK protein suppresses lipopolysaccharide-induced acute lung injury in rats
- Characterization of the degradation of recombinant rat urate oxidase in tetracycline controlled gene expression cells
- High-Pressure Brillouin Study on Deuterated Ammonia III
- Three-Magnon Linewidth and Effective Exchange Constant in a Hexagonal Ferrite, Zn_2Y
- Specific Role of T and Tn Tumor-associated Antigens in Adhesion between a Human Breast Carcinoma Cell Line and a Normal Human Breast Epithelial Cell Line
- Specific Role of T and Tn Tumor-associated Antigens in Adhesion between a Human Breast Carcinoma Cell Line and a Normal Human Breast Epithelial Cell Line
- Antisense oligodeoxynucleotides against thrombomodulin suppress the cell growth of lung adenocarcinoma cell line A549
- Superconductivity and Magnetic Properties of YBa_2(Cu_Fe_x)_3O_
- Psychosocial Factors That Have an Influence on the Effects of Obesity Improvement Programs
- Observation of Narrow Structures in the P-P Analyzing Powerand Narrow Resonances
- Two-Magnon Linewidth Induced by Multipole Demagnetizations of Surface Imperfections on Zn_2Y
- Angiogeneis in the alveolar walls with neoplastic cell spreading of primary lung carcinomas
- Strong Correlation among Structural, Electronic, and Magnetic Properties of Sr2Fe1+xMo1-xO6 (0 \leq x \leq 1)
- The endothelium of the pulmonary microvessels
- Interphase cytogenetic analysis of lung adenocarcinomas with bronchioloalveolar pattern
- Magnetic Properties of Nd_Ce_xCuO_Studied by Doped Gd^-EPR
- Disseminated Cryptococcosis Complicated with Bilateral Pleural Effusion and Ascites during Corticosteroid Therapy for Organizing Pneumonia with Myelodysplastic Syndrome
- Supplementation of Heterologous Complement Induces Anti-Thy-1.1 Nephritis in the Mongolian Gerbil (Meriones unguiculatus).
- PRINCIPAL COMPONENTS REGRESSION BY USING GENERALIZED PRINCIPAL COMPONENTS ANALYSIS