高遠液体クロマトグラフィーによる尿中ピペミド酸の定量およびその薬動力学

概要

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A high performance liquid chromatography (HPLC) was used for the determination of urinary pipemidic acid (PPA). PPA was chromatographed as ion pairs with ethanesulphonic acid by use of Fine pack SIL C_<18> 10 reverse phase column. The amount of PPA was determined by the peak height ratio. The coefficients of variation at within-run were 1.77% (50μg/ml) and 2.83% (1200μg/ml), and at between-run were 8.77% (50 μg/ml) and 3.34% (1200 μg/ml). The urinary levels of PPA after oral administration of 500 mg to healthy male subjects were measured and pharmacokinetics o f PPA were studied. The mean elimination rate constant (ke) was 0.27±0.02 h^<-1>, biological half-life (t_<l/2>) was 2.6±0.16 h, and the maximal time of the elimination rate (T_<MAX>) was 2.5±1 h. It was confirmed that the urinary levels of PPA determined by this method agree well with those by bioassay.
日本医療薬学会の論文
1985-06-20