Determination of concetrations of antiepileptic agents in saliva by fluorescence polarization immunoassay technique.

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We intended to determine the concentrations of antiepileptic agents in saliva, as a substitute of serum, by the fluorescence polarization immunoassy technique, using the TDx analyzer (Dainabot). It had been considered that it would be difficult to handle a very small amount of saliva samples (2-10 μl) with the TDx analyzer because of its high viscosity. However, the drug concentrations in saliva could be determined by the analyzer after the saliva sample was diluted with an equal volume of saline. Using the salivary concentrations determined by this technique, we could obtain the simple and practical equations, which correlate the total concentrations of drugs in serum (Ct) with those in saliva (Cs) without taking the salivary pH into consideration as follows: 1) phenytoin (PHT): Ct=9. 422 Cs, r=0. 965, n=5; 2) phenobarbital (PB): Ct=4.261 Cs, r = 0.883, n=27; 3) carbamazepine (CBZ): Ct=3. 200 Cs, r= 0.734, n=10. As for sodium valproate (VPA), we could predict the maximal saturated concentration of the bound drug (Cb) with serum protein as 102 μg/ml from double-reciprocal plot between the bound and free (Cf) concentrations in serum as follows: 4) sodium valproate: Cb-1=0.0575 Cf-1 +0.00981, r= 0.850, n=38. And this maximal value of Cb would indirectly support the upper therapeutic range (about 100 μg/ml) of VPA in Japanese. However, the above equations must be reconstructed in each laboratory, because they were obtained from a small population.
一般社団法人日本臨床薬理学会の論文

Determination of concetrations of antiepileptic agents in saliva by fluorescence polarization immunoassay technique.

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