高速液体クロマトグラフィーによるフィゾスチグミンの定量法と水溶液中の安定性

概要

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A high performance liquid chromatographic (HPLC) method was developed for the determination of physostigmine (PS) in the presence of degradation products, and the stability of PS in aqueous solution was also examined by this method. Sufficient separation was effected on an ODS column with an eluent of CH_3CN : 0.5% CH_3 COONH_4 aq. (70 : 30). Tolperisone HCl was most suitable of all drugs studied for the internal standard. Coefficient of variation for determination of PS was less than 1%. HPLC method was better, in its simplicity and accuracy, than other methods (U. S. P. methods etc.). PS in buffered aqueous solution was most stable at about pH 3, when heated in boiling water. Although PS solution in anaerobic condition was almost colorless and produced rubreserine and unknown decomposed substance not recognized in aerobic condition, no difference could be detected for the degradation rate of PS. It was found that N_2 exchange retards the discoloration, but it has no effect on the stability of PS solution.
日本医療薬学会の論文
1983-08-20