逆相分配イオンペア高速液体クロマトグラフィーによる血清中テオフィリンの定量

概要

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A new specific high-performance liquid chromatographic method for the determination of theophylline (TPH) in human serum was studied. In this method, TPH in serum was extracted with chloroform-isopropanol (7 : 3) ; then, an aliquot of the extract was evaporated to dryness in vacuo. TPH was determined by an ion-pairing reversed-phase high-performance liquid chromatography using a Zorbax-ODS column. The mobile phase was a mixture of acetonitrile water (5 : 95 by vol) containing 10 mM sodium acetate and 3 mM tetrabutylammonium hydrogen sulfate (pH 6.0). Beta-hydroxyethyltheophylline was used as an internal standard. This method enables to separate TPH and paraxanthine, a metabolite of caffeine which interferes with most TPH assay procedures.
日本医療薬学会の論文
1984-06-20