ダイオキシンのマウス生殖細胞への影響 : シンポジウム : II. ダイオキシンの生体影響

概要

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DNA damage induced in male germ cells by 2,3,7,8-tetrachloro dibenzo-p-dioxin (TCDD) and its repair were measured in C3H/HeN mice. Aithough about 1/25 of TCDD in the liver was found in the testis after the intraperitoneal injection, unscheduled DNA synthesis (UDS) and single-strand breaks were not detected at all stages of male germ cells. Chromosome aberrations in the M1 stage of spermatocytes were also not detected. After the direct injection of TCDD into the testis, I amounts of TCDD bound to the sperm head were maximum at the 7th day after the iniection, and minor UDS were detected at stages of spermatogonia, spermatocytes and early spermatids. In contrast to the negligible DNA damage to the male germ cells, degeneration of endoplasmic reticulum and condensation of nuclei were commonly observed in the Sertoli cells (nursing cells of male germ cells) of TCDD-treated mice. Degeneration of Sertoli cells becomes more serious with the time elapsed after exposure, resulting in the indirect killing of male germ cells. (This paper, in a session chaired by Taisei Nomura and Kazuo Hujikawa, was presented to the sympo-sium "Biological effects of dioxins", organized by Taisei Nomura, at the 27+" annual meeting of the Environmental Mutagen Society of Japan, and held at the Mielparque Osaka in Osaka, Japan, Novem ber, 24-26, 1998.)
日本環境変異原学会の論文
1999-07-12