画像処理による長焦点深度光学顕微鏡画像の合成 : 長焦点深度画像の合成と三次元表示

概要

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A original method to get long depth of focus of optical microscope image using computer image processing has been proposed. At first the principle of proposed method is explained, then the validity of the method is shown experimentally. The microscope objective is determined positioning by actuator such as PZT. At each located position digital picture image through CCD camera is taken into a computer. The computer extracts the area where the image is in focus from these pictures, then it composes whole frame image. The depth of focus of the composite picture image is nearly equal to the distance of motion of objective lens. As for the methods that distinguish between in-focus-area and out-of-focus-area, four different methods have been proposed. Processing time and accuracy point of view Hadamard transfer method is the best within these four methods. This method has been applied to the observation of several specimens such as standard surface roughness test piece, grooved metal surface and so on, with excellent results. By adding displacement information to the recorded CCD camera image, this method is also applicable to the three dimensional surface topography measurement.
公益社団法人精密工学会の論文
1994-08-05