A Nobel Serine Protease Produced by Marine Isolated Strain of Vibrio anguillarum
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概要
- 論文の詳細を見る
A serine protease was purified to homogeniety from the culture supernatant of a marine bacterium, Vibrio anguillarum by a 4-step procedure, ammonium sulfate precipitation, G-100 gel filtration, and two rounds of phenyl-Sepharose chromatography. The molecular weight of the enzyme was estimated to be 30 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration. The enzyme activity was inhibited by diisopropyl fluorophosphate, phenylmethyl sulfonyl fluoride, Leupeptin, and Chymostatin. The optimum temperature was 37℃ and the optimum pH of the enzyme was 9.0. Mg^<2+> and Ca^<2+> at 10 mM enhanced the enzyme activity by 161 and 124 %. And stabilized the activity when exposed between 15 to 42℃. The enzyme hydrolyzed strongly butyloxycarbonyl-leucyl-seryl-threonyl-arginyl-4-methylcoumaryl-7-amide among 17 peptydyl-4-methylcoumaryl-7-amides, indicating narrow substrate specificity. The alkaline serine enzyme shows similar substrate specificity to a known metalloprotease. However, this novel serine protease did not show anticoagulation activity as detected in the metalloprotease, suggesting a different function in this bacterium.
- 日本微生物生態学会の論文
- 1997-12-31
著者
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Suzuki Satoru
Department of Aging Medicine and Geriatrics, Shinshu University, Graduate School of Medicine
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鈴木 聡
高知大学農学部栽培漁業学科海洋生物育成学講座
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楠田 理一
福山大学海洋生物工学科
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MORITA JUN
United Graduate School of Agricultural Sciences, Ehime University
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KUSUDA RIICHI
Department of Aquaculture, Kochi University
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楠田 理一
Department Of Marine Biotechnology Fukuyama University
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Kusuda Riichi
Fish Disease Laboratory Department Of Aquaculture Kochi University
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Kusuda Riichi
Department Of Aquaculture And @s12@e1research Institute For Molecular Genetics Kochi University
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Morita J
United Graduate School Of Agricultural Sciences Ehime University:department Of Aquaculture Kochi Uni
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Morita Jun
United Graduate School Of Agricultural Sciences Ehime University:department Of Aquaculture Kochi Uni
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Suzuki Satoru
Department of Aging and Geriatrics, Shinshu University, Graduate School of Medicine
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