線維化進展度の測定 : ハイドロキシプロリンのLC/MSによる新測定法

概要

論文の詳細を見る
We investigated a highly sensitive method to determine the degree of fibrosis of various organs by Liquid chromatography/Mass Spectrometry (LC/MS). The results suggested that the method of LC/MS for determining hydroxyproline had a significantly higher sensitivity than colorimetric and fluorescent labelling methods. In addition, the effect of complex amino acid components from living body samples mixing with hydroxyproline was eliminated. According to the peak observed on the chromatography findings, the molecular structure of hydroxyproline could be accurately identified. Therefore, we concluded that the new method of LC/MS was very useful for measuring hydroxyproline in living body samples containing large amounts of complex amino acid components.
日本医療薬学会の論文
2003-08-10