カラムスイッチングHPLCシステムによるゾニサミドの高感度測定法の評価

概要

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A fully automated high-performance liquid chromatography system with column-switching was evaluated to determine the concentration of zonisamide (ZNS) in human plasma. After the plasma sample was diluted to 10-times with the mobile phase, the sample (10μL) was injected into the column-switching system without pretreatment. ZNS was detected by ultraviolet absorption at 246 nm. The calibration curve of ZNS was linear and ranged from 1.57 to 50μg/mL of plasma. The coefficients of variation in the within-run (n=5) and the between-run (5 days) precisions of ZNS were below 5 %. The limit of quantification was 0.5μg/mL in 1μL of human plasma. A good correction was found between the data obtained by this system and the ELISA system using Markil-M Excegran (r=0.956) or the solid-phase-xtraction HPL.C method (r=0.985). This system for ZNS is thus considered to be a very useful measurement for therapeutic drug monitoring because or its high sensitivity, rapidity and low cost compared with other measurement systems.
日本医療薬学会の論文
2003-04-10