ラットの骨および象牙質の多重蛍光ラベリング像の共焦点レーザースキャン顕微鏡による観察

概要

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Polychromatic fluorescent labeling of bone and dentin was observed by confoca1 1aser scanning microscopy. Male Wistar rats (body weight 100g) were labeled with multiple injections of fluorchromes, tetracyc11ne, calcein and a1izarin red S at a dose of 2 mg/kg, 3mg/kg and 4 or 8 mg/kg respectively. The time intervals of each labeling was 3 or 4 days. Undecalciried ground sections (thickness, 100μm) of cortical bone of tibia and mandibular incisor were prepared. Polychromatic fluorescent labeling lines of calcein and alizarin red S were clearly observed by Ar-1aser emission 488nm, but tetracycline fluorescence was weakly labeled the bone and dentin. The clear image of fluorescent labeling pattern was obtained from the optical section through thick specimen by the confocal mode of the microscopy. For the confocal laser scanning microscopy, calcein and alizarin red S are the most suitable markers for double fluorescent labeling of bone and dentin and produce good results. Tetracycline seems to be unsuitable as a marker for this purpose.
硬組織再生生物学会の論文
1993-10-31