家蚕消化液赤色螢光蛋白の生合成について

概要

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To investigate the biosynthesis off the red fluorescent protein which is considered to possibly play some protective role against polybedroses, comparative experiments with the larvae, reared on mulberry leaves and artificial diets, were conducted by four methods : a) column-chromatography, b) DISC-electrophoresis, c) incorporation of the C^<14>-Leucine, d) immunological method. In larvae fed on mulberry leaves the red fluorescent protein was formed in the anterior part of the mid-gut and secreted from there into the digestive juice. In larvae fed on artificial diet this protein was found too, but the red fluorescence of this protein was not observed as expected. The red fluorescence strength of the protein from the latter, estimated at 625 mμ by a fluorometer, appeared with the addition of fresh chloroplast fraction of spinach to the diet. This protein seemed to be a conjugated protein bearing certain bile pigments similar to the non-proteinous part attributed to mulberry leaves in the diet. From these results, it is highly probable that this protein is biosynthesized de novo in the mid-gut tissue of the silkworm and not from certain precursors contained in the mulberry leaves of the diet.
1971-09-25