カイコ幼虫消化液中の赤色けい光たんぱく質(RFP)の生成機構 : クロロフィリド-a-中腸たんぱく質複合体の形成

概要

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The biliprotein RFP which was present in the digestive juice of the silkworm larvae, was biosynthesized in vitro by mixing and incubating the two components. One of the components was chlorophyllide-a (Ch-a) and the other was a midgut protein (MP) which was extracted and purified from the midgut of the silkworm larvae. This biosynthesis system involved sequential reactions. The first reaction consisted of the formation of a bond between Ch-a and MP and the formation of a Ch-a-MP complex. This reaction did not require either oxygen or light. In the second reaction the porphyrin ring of this complex was cloven and converted into a bilepigment, and then RFP was biosynthesized. This reaction required both oxygen and light. Therefore, it was assumed that the bond was photooxidized to a chromophore of RFP.
1981-05-25