Deoxyribonuclease activity in Bacillus subtilis var. amyloliquefacus cells infected with bacteriophages

概要

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DNase activity in Bacillus subtilis var. amyloliquefacus cells infected with phage F or phage S-a was examined. Plaque-forming efficiencies of these two phages on this host were almost identical (1 and 1.1,respectively). The infection of phage F caused a remarkable increase in DNase activity having an optimal pH at about 9.5,but the infection of phage S-a did not. The DNase activities on native and heat-denatured DNA at 35 min after infection of phage F reached approximately 9- and 35-fold of the activities in uninfected cells, respectively. The increase in this enzyme activity was due neither to the destruction of inhibitors nor to the synthesis of stimulators, and was completely prevented by chloramphenicol. This kind of DNase was not found in uninfected cells. It was clarified that the increase in DNase activity observed after phage F infection is due to the synthesis of a new DNase induced by the infection. This enzyme will be referred to "phage F-induced DNase".
日本医科大学の論文
1986-03-01