<基礎的研究発生病理>胸髄中間外側部アセチルコリン性ニューロンへのアドレナリン性入力の形態学的検討

概要

論文の詳細を見る
Synaptic relationships of adrenergic afferents to the preganglionic cholinergic neurons in the rat thoracic spinal cord were examined using a peroxidase/β-galactosidase double-immunostaining method for light and electron microscopy. Cholinergic neurons were labeled by a monoclonal antibody to choline acetyltransferase and stained bluish green by 5-bromo-4-chloro-3-indolyl-β-D-galactoside reaction products using β-galactosidase, whereas adrenergic fibers were labeled by a polyclonal antibody to phenylethanolamine-N-methyltransferase and stained brown by diaminobenzidine reaction products using peroxidase. As a result, the preganglionic neurons in the thoracic spinal cord were found to receive direct synaptic inputs of adrenergic projection from putative origins in the adrenergic C1 group involved in a vasopressor function.
近畿大学の論文
1992-12-30