Lactoferrin Inhibits the Acquisition of Dry-Resistance of Salmonella spp.
スポンサーリンク
概要
- 論文の詳細を見る
An assay method was established for estimation of dry-resistance of Salmonella. Environmental isolates of Salmonella enterica spp., including S. Enteritidis were grown to the logarithmic phase, washed and re-suspended in saline or Luria-Bertani (LB) medium, followed by drying overnight in an automatic dry-keeper at room temperature. The dried bacteria were recovered by mixing with ice-cold PBS, suspended, and examined for viability by colony-forming activity. A pathogenic clone of S. Enteritidis, SECl#15-1, was not viable in saline alone but maintained its viability in LB medium, suggesting it requires nutrients for the acquisition of dry-resistance. Addition of lactoferrin or apolactoferrin to the bacterial suspension in 20% LB medium prior to the dry-protocol decreased the viability of SECl#15-1 in a dose-dependent manner. However, lactoferrin showed no effect on the growth of SECl#15-1 in liquid culture with LB or M9 medium, suggesting that it exerts bactericidal effects under dry but not under wet conditions. Besides, Salmonella spp. other than S. Emteritidis, such as S. Typhimurium, S. Oranienburg, S. Weltevreden, S. Johannesburg, and S. Infantis, also showed dry-resistance, which was significantly inhibited by lactoferrin and almost entirely by apolactoferrin. These results suggest that lactoferrin inhibits the acquisition of dry-resistance by Salmonella spp., suggesting that there is a possible use for lactoferrin in the control of Salmonella food-poisoning as an additive in dry food.
- 2009-07-01
著者
-
IGIMI Shizunobu
Division of Biomedical Food Research, National Institute of Health Sciences
-
AMANO Fumio
Laboratory of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences
-
Amano Fumio
Laboratory Of Biodefense & Regulation Osaka University Of Pharmaceutical Sciences
-
Amano Fumio
Lab. Of Biodefense & Regulation Osaka Univ. Of Pharmaceutical Sciences Jpn
-
Igimi Shizunobu
Division Of Biomedical Food Research National Institute Of Health Sciences
-
Igimi S
Division Of Biomedical Food Research National Institute Of Health Sciences
-
Igimi Shizunobu
Division Of Biomedical Food Research National Institute Of Health Sciences;
-
TAMURA AI
Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences
-
Tamura Ai
Laboratory Of Biodefense & Regulation Osaka University Of Pharmaceutical Sciences
-
NISHIO Erika
Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences
-
FUJIMORI Ko
Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences
-
Fujimori Ko
Laboratory Of Biodefense & Regulation Osaka University Of Pharmaceutical Sciences
-
Nishio Erika
Laboratory Of Biodefense & Regulation Osaka University Of Pharmaceutical Sciences
-
Amano F
Laboratory Of Biodefense & Regulation Osaka University Of Pharmaceutical Sciences
関連論文
- Hazard Analysis of Listeria monocytogenes Contaminations in Processing of Salted Roe from Walleye Pollock (Theragra chalcogramma) in Hokkaido, Japan(Public Health)
- Effects of CpG-DNA from Escherichia coli on Digoxin Pharmacokinetics(Biopharmacy)
- Changes in Digoxin Pharmacokinetics Treated with Lipopolysaccharide in Wistar Rats(Biopharmacy)
- Effects of Capsaicin on Cellular Damage and Monolayer Permeability in Human Intestinal Caco-2 Cells(Biopharmacy)
- Dry-Resistance of Salmonella enterica subsp. enterica Serovar Enteritidis is Regulated by Both SEp22, a Novel Pathogenicity-Related Factor of Salmonella, and Nutrients
- An Anti-Salmonella Antibody Prevents the Salmonella enterica serovar Enteritidis from Infecting the Human Intestinal Epithelial Cell Line, Caco-2, by Interacting with Flagella
- Isolation of Staphylococcus aureus from Raw Fish in Relation with Culture Methods
- Proteomic Characterization of Enterohemorrhagic Escherichia coli O157:H7 in the Oxidation-Induced Viable but Non-Culturable State
- Lactoferrin Inhibits the Acquisition of Dry-Resistance of Salmonella spp.
- Endotoxin-Tolerance to the Cytotoxicity toward a Macrophage-Like Cell Line, J774.1, Induced by Lipopolysaccharide and Cycloheximide: Role of p38 MAPK in Induction of the Cytotoxicity(Highlighted paper selected by Editor-in-chief)(Biochemistry/Molecular Bi
- Enhancement of Mice Susceptibility to Infection with Listeria monocytogenes by the Treatment of Morphine
- Isolation of Mini-Tn5Km2 Insertion Mutants of Salmonella enterica Serovar Oranienburg Sensitive to NaCl-Induced Osmotic Stress
- Regulation of SEp22 Expression in Salmonella enterica subsp. enterica Serovar Enteritidis by Culture Medium
- Diverse Pathogenicity of Salmonella Enteritidis Clones Isolated from Poultry Farms in Chicks and BALB/c Mice
- Effects of Carrageenans on the Binding, Phagocytotic, and Killing Abilities of Macrophages to Salmonella(Food & Nutrition Science)
- Genetic Characterization of Thermal Tolerance in Enterobacter sakazakii
- Enhanced Release of Prostaglandin D_2 during Re-incubation of RAW 264.7 Macrophage-Like Cells after Treatment of Both Lipopolysaccharide and Non-steroidal Anti-inflammatory Drugs(Biochemistry/Molecular Biology)
- Antimicrobial Susceptibilities of Listeria monocytogenes Isolated in Japan
- Effects of Anaerobic Preculture on Aerobic Stress Responses of Campylobacter jejuni
- Structural Studies on Strained-Layer Lattices
- The Occurrence of Listeria monocytogenes in Imported Ready-to-Eat Foods in Japan
- The Occurrence of Listeria monocytogenes in Imported Ready-to-Eat Foods in Japan
- Development of Recombinant Vaccines in Lactobacilli for Elimination of Salmonella
- Regulatory role of cardiolipin in the activity of an ATP-dependent protease, Lon, from Escherichia coli