Transplantation of neural progenitors derived from embryonic stem cells brings about functional and electrophysiological recoveries of mice with spinal cord injury
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概要
- 論文の詳細を見る
Cell transplantation is promising therapeutic strategy for spinal cord injury (SCI). Embryonic stem (ES) cells proliferate in vitro through many passages without losing their totipotentiality and are good sources for transplantable cells. However, whether the grafted cells further differentiate into mature motoneurons and directly contribute to the reconstruction of neuronal pathway in SCI remains largely unknown. In this study, mouse ES cells were treated with retinoic acid to induce neural progenitor cells, which were transplanted to the completely transected spinal cord at T7-T8 of mice. The mice transplanted with neural progenitor cells exhibited potent functional recoveries of their hind limbs and tail, showing significant higher scores of locomotor behavioral tests compared with SCI mice having vehicle injection, and electrophysiological assessment confirmed the re-emergence of motor evoked potential of injured spinal cord. Histological analysis of injured spinal cord demonstrated that engraftment of the neural progenitor cells into a vacant spinal column cavity made by removal of the transected spinal cord, reconnected the spinal cord and large numbers of grafted cells were found alive. The grafted cells expressed neurofilament middle chain and Synaptophysin, suggesting differentiation into mature neurons and synapse formation. RT-PCR and immunohistochemical analyses demonstrated that the differentiated neurons expressed markers for motoneurons, Islet1, HB9 and Lim1/2, suggesting their differentiation into spinal motoneurons. Taken together, neural progenitor cells derived from ES cells can be successfully transplanted to injured spinal cord, survive, further differentiate into motoneurons and reconstruct neuronal pathways in vivo, resulted in functional and electrophysiological recoveries of injured spinal cord.
- 日本炎症・再生医学会の論文
- 2004-11-25
著者
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Suzuki Noboru
Department of Advanced Interdisciplinary Sciences, Graduate School of Engineering, Utsunomiya Univer
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KUROKAWA Manae
Department of Immunology and Medicine, St. Marianna University School of Medicine
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CHIBA Shunmei
Department of Immunology and Medicine, St. Marianna University School of Medicine
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榊原 学
東海大学開発工学部生物工学科
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Chiba Shunmei
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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Suzuki Noboru
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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Kurokawa Manae
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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榊原 学
東海大 開発工
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Watanabe Kenji
Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute
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Aoki Haruhito
Department Of Orthopaedic Surgery St. Marianna University School Of Medicine
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Aoki Haruhito
Department Of Orthopedic Surgery St. Marianna University School Of Medicine
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YOSHIKAWA Hideshi
Department of Parasitology and Immunology, Yamanashi Medical University
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Akashi K
Department Of Emergency Critical Care Medicine St. Marianna University School Of Medicine
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Akashi Katsuya
Department Of Acute Medicine St. Marianna University School Of Medicine
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MASUDA Chieko
Department of Immunology and Medicine, St. Marianna University School of Medicine
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Sakakibara Manabu
Laboratory Of Neurobiological Engineering School Of High-technology For Human Welfare Tokai Universi
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Takada Erika
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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Masuda Chieko
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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HAMADA Mari
Department of Immunology and Medicine, St. Marianna University School of Medicine
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Hamada Mari
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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Yoshikawa Hideshi
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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Suzuki Noboru
Department Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya Universi
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Watanabe Kenji
Department Of Immunology And Medicine St. Marianna University School Of Medicine
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Watanabe Kenji
Laboratory Of Regeneration Biology Department Of Life Science Graduate School Of Science Himeji Inst
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Watanabe Kenji
Laboratory Of Food Material Engineering Faculty Of Agriculture Gifu University
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