Protein adsorption on needle-shaped hydroxyapatite prepared by hydrothermal treatment of mixture composed of CaHPO_4・2H_2O and β-Ca_3(PO_4)_2

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概要

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• The behavior of protein adsorption on needle-shaped hydroxyapatite (HAp) crystals was investigated and compared with that on irregularly shaped HAp crystals in phosphate buffer solutions. The proteins used were bovine serum albumin (BSA) and lysozyme chloride (LSZ). The effect of phosphate ions in the buffered solutions was evaluated at concentrations in the range 2-200 mol·m-3. In 50 mol·m-3 or lower buffer concentrations, the saturated amount of BSA adsorbed on needle-shaped HAp was greater than that on irregularly shaped HAp. The saturated amount of BSA adsorbed on needle-shaped HAp decreased with increasing phosphate buffer concentration. This resulted in little difference in BSA adsorption between the needle-shaped HAp and the irregularly shaped HAp at phosphate concentrations of 100 mol·m-3 or higher. In contrast, the saturated amount of adsorbed LSZ increased with increasing phosphate buffer concentrations up to 10 mol·m-3 and subsequently decreased with increasing phosphate concentrations between 10 and 200 mol·m-3. There were no differences in the protein adsorption behaviors between the needle-shaped HAp and irregularly shaped HAp at a phosphate concentration of 200 mol·m-3. A specific adsorption, useful for the separation of BSA and LSZ proteins was observed for the needle-shaped HAp at a low phosphate buffer concentration. This separation was caused by the large a-face surface area of the needle-shaped HAp by comparison with that of the irregularly shaped HAp.

• 2009-07-01

著者

• KAMITAKAHARA Masanobu

  Graduate School of Environmental Studies, Tohoku University

• OHTSUKI Chikara

  Graduate school of Engineering, Nagoya University

• Kamitakahara Masanobu

  Graduate School Of Environmental Studies Tohoku University

• Kamitakahara Masanobu

  Graduate School Of Environmental Studies Tohoku Univ.

• Ohtsuki Chikara

  Department Of Crystalline Materials Science Graduate School Of Engineering Nagoya University

• Ohtsuki Chikara

  Graduate School Of Engineering Nagoya Univ.

• Ohtsuki C

  Department Of Crystalline Materials Science Graduate School Of Engineering Nagoya University

• Ohtsuki Chikara

  Dep. Of Crystalline Materials Sci. Graduate School Engineering Nagoya Univ.

• Ohtsuki Chikara

  奈良先端科学技術大学院大学 物質創成科学研究科

• KAWACHI Giichiro

  Department of Biomaterials, Faculty of Dental Science, Kyushu University

• Ohtsuki Chikara

  Graduate School Of Engineering Nagoya University

• OGATA Shin-ichi

  Graduate School of Environment and Information Sciences, Yokohama National University

• KAWACHI Giichiro

  Graduate School of Engineering, Nagoya University

• WATANABE Tomoko

  Graduate School of Engineering, Nagoya University

• Ogata Shin-ichi

  Graduate School Of Environment And Information Science Yokohama National University

• Ogata Shin-ichi

  Graduate School Of Enviroment And Information Science Yokohama National University

• Watanabe Tomoko

  Graduate School Of Engineering Nagoya University

• Ogata Shin-ichi

  Graduate School Of Bioscience And Biotechnology Tokyo Institute Of Technology

• Ogata Shin-ichi

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

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