Isolation and Culture of Rabbit Primordial Germ Cells
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概要
- 論文の詳細を見る
Primordial germ cells (PGCs) are embryonic precursors of the gametes of adult animals and are considered stem cells of the germline. Since their proliferation in vitro correlates well with the schedule of developmental changes in vivo, they might be interesting research tools for genomic imprinting, germ-cell tumors and fertility. Furthermore, once primordial germ cells are separated and placed on a feeder layer with cytokines, they become cultured pluripotent cell lines called embryonic germ (EG) cells. EG cells share several important characteristics with embryonic stem (ES) cells as they can also contribute to the germ line of chimeras. To investigate the characteristics of PGCs and establish rabbit EG (rEG) cells, we cultured rabbit PGCs (rPGCs) in vitro with various combinations of leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and forskolin on inactivated mouse embryonic fibroblast (MEF) feeder layers. The present study found PGC proliferation in early cultures and induction of rEG-like colonies. These cells expressed pluripotent markers, such as alkaline phosphatase activity, OCT-4, Sox-2 and SSEA-1, in the undifferentiated state; however, the cells did not develop into a teratoma when injected into the kidney capsules of SCID mice, although the restricted differentiation potentials to neural cells were determined via embryoid body formation. From these characteristics and further characterization of the germ stem cell markers Vasa, SCP-1 and SCP-3, we suggested that these were hybrid cells with characteristics somewhere between PGC and EG cells.
- 日本繁殖生物学会の論文
- 2008-10-01
著者
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Matsumoto Kazuya
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Matsumoto Kazuya
Graduate School University Of East Asia
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MITANI Tasuku
Institute of Advanced Technology, Kinki University
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ANZAI Masayuki
Institute of Advanced Technology, Kinki University
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Fukuda Kanji
Institute Of Advanced Clinical Medicine Kinki University School Of Medicine
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Saeki K
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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SAGAWA NORIMASA
Department of Obstetrics and Gynecology, Mie University Graduate School of Medicine
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Hosoi Y
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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TERAMURA Takeshi
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University
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KAKEGAWA Ryo
Graduate School of Biology-Oriented Science and Technology, Kinki University
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TAKEHARA Toshiyuki
Graduate School of Biology-Oriented Science and Technology, Kinki University
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SAEKI Kazuhiro
Graduate School of Biology-Oriented Science and Technology, Kinki University
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HOSOI Yoshihiko
Graduate School of Biology-Oriented Science and Technology, Kinki University
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Hosoi Yoshihiko
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Mitani Tasuku
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Mitani Tasuku
Institute Of Advanced Technology Kinki University
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Anzai Masayuki
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Sagawa Norimasa
Department Of Obstetrics And Gynecology Graduate School Of Medicine Mie University
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Sagawa Norimasa
Department Of Gynaecology And Obstetrics Kyoto University Graduate School Of Medicine
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Kakegawa Ryo
Graduate School Of Biology-oriented Science And Technology Kinki University
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Matsumoto Kazuya
徳島大学医学部
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Teramura Takeshi
Department Of Obstetrics And Gynecology Graduate School Of Medicine Mie University
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Anzai Masayuki
Institute Of Advanced Technology Kinki University
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Takehara Toshiyuki
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Matsumoto Kazuya
Graduate School Of Computer Science And Engineering The University Of Aizu
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Hattori Yoshihisa
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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TERAMURA Takeshi
Department of Biology-Oriented Science and Technology, Division of Genetic Engineering, Kinki University
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