Identification of ZAG1, a Novel Protein Expressed in Mouse Preimplantation, and Its Putative Roles in Zygotic Genome Activation
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概要
- 論文の詳細を見る
We isolated a mouse cDNA, zag1 (zygotic gene activation-associated gene 1), that has an open reading frame of 1728-bp encoding a protein of 66.2 kDa including both a bipartite nuclear targeting sequence and a P-loop motif containing nucleoside triphosphate hydrolase motifs. Northern blot analysis of mouse tissues showed that zag1 was widely expressed but was especially prominent in the ovary and testis. RT-PCR analysis of in vitro fertilized embryos showed that the abundance of zag1 transcripts in oocytes decreased after fertilization, and zag1 mRNA was detected at 15 h post insemination (hpi) in fertilized embryos indicating that the gene was expressed at the start of zygotic gene activation at the mouse 1-cell stage. The nuclear-localization of ZAG1 protein in mouse preimplantation embryos at 15 hpi was confirmed by both subcellular analysis of enhanced green fluorescent protein (EGFP)-tagged ZAG1 and immunocytochemical analysis with anti-ZAG1 antibody. Subsequently, using yeast two-hybrid screening, we identified U2 small nuclear ribonucleoprotein B (U2B"), which is associated with pre-mRNA splicing, as a putative interacting partner of ZAG1 protein. Furthermore, knockdown of zag1 expression by an antisense DNA plasmid induced arrest and/or delay of embryonic development in injected 1-cell embryos. These results suggest that ZAG1 may be closely associated with zygotic gene expression in mouse preimplantation embryos.
- 日本繁殖生物学会の論文
- 2008-06-01
著者
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TOKORO Mikiko
Division of Biological and Science, Graduate School of Biology-Oriented Science and Technology, Kink
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SHIN Seung-Wook
Division of Biological and Science, Graduate School of Biology-Oriented Science and Technology, Kink
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SAEKI Kazuhiro
Division of Biological and Science, Graduate School of Biology-Oriented Science and Technology, Kink
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HOSOI Yoshihiko
Division of Biological and Science, Graduate School of Biology-Oriented Science and Technology, Kink
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IRITANI Akira
Division of Biological and Science, Graduate School of Biology-Oriented Science and Technology, Kink
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MATSUMOTO Kazuya
Division of Biological and Science, Graduate School of Biology-Oriented Science and Technology, Kink
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Iritani Akira
Division Of Biological And Science Graduate School Of Biology-oriented Science And Technology Kinki
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Tokoro Mikiko
Division Of Biological And Science Graduate School Of Biology-oriented Science And Technology Kinki
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Tokoro Mikiko
近畿大学 生物理工学研究科
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Shin Seung-wook
Division Of Biological And Science Graduate School Of Biology-oriented Science And Technology Kinki
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Shin Seung-wook
近畿大学 生物理工学研究科
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Matsumoto Kazuya
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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AMANO Tomoko
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki Un
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MITANI Tasuku
Institute of Advanced Technology, Kinki University
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KATO Hiromi
Institute of Advanced Technology, Kinki University
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ANZAI Masayuki
Institute of Advanced Technology, Kinki University
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Sato Manabu
Division Of Cardiovascular Surgery Nagasaki Kouseikai Hospital
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Iritani A
Division Of Biological And Science Graduate School Of Biology-oriented Science And Technology Kinki
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Iritani Akira
Laboratory Of Animal Reproduction Department Of Animal Science Kyoto University
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Saeki K
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Sato Manabu
The Centre For Reproductive Medicine And Infertility Ivf Namba Clinic
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MATSUOKA Toshiki
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki Un
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UENOYAMA Atsuto
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki Un
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ITO Kazunari
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki Un
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HITOMI Syuji
Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki Un
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Hitomi Syuji
Division Of Biological Science Graduate School Of Biology-oriented Science And Technology Kinki Univ
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Ito Kazunari
Division Of Biological Science Graduate School Of Biology-oriented Science And Technology Kinki Univ
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Uenoyama Atsuto
Division Of Biological Science Graduate School Of Biology-oriented Science And Technology Kinki Univ
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Hosoi Y
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Hosoi Yoshihiko
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Kato Hiromi
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Amano Tomoko
Division Of Biological Science Graduate School Of Biology-oriented Science And Technology Kinki Univ
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Mitani Tasuku
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Mitani Tasuku
Institute Of Advanced Technology Kinki University
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Anzai Masayuki
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Matsumoto Kazuya
徳島大学医学部
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Kato Hiromi
Institute Of Advanced Technology Kinki University
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Matsuoka Toshiki
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Anzai Masayuki
Institute Of Advanced Technology Kinki University
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Hattori Yoshihisa
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Sato Manabu
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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