Timing and Uniformity of Embryonic Gene Activation Affect Subsequent Pre-implantation Development of Cloned Bovine Embryos
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概要
- 論文の詳細を見る
In the present study, we examined the timing of onset, intensity, and mosaicism of embryonic gene expression in bovine nuclear transfer (NT) embryos. The relationship between gene expression and early embryonic development was also examined. To monitor the gene expression of NT embryos, we produced NT embryos with bovine transfected fibroblasts carrying a firefly luciferase gene under the control of a chicken β-actin promoter, an expression system that has previously been shown to be representative of embryonic gene expression in mice. Photon count imaging showed that luciferase luminescence began in NT embryos with fibroblasts 48 hours post fusion (hpf) and reached a plateau at the 4- to 8-cell stage at 60 hpf. Only 4- to 8-cell NT embryos luminescent by 60 hpf developed to the blastocyst stage. At 60 hpf, strongly luminescent embryos developed to the blastocyst stage at a higher rate (P<0.05) than embryos with weak or absent luminescence. However, embryos with mosaic luminescence developed at a much lower rate (P<0.05) than those with whole-embryo luminescence, even if the embryos exhibited strong luminescence. Our results indicate that precise and uniform embryonic gene expression at the 4- to 8-cell stage at 60 hpf may be closely related to development of bovine NT embryos to the blastocyst stage.
- 日本繁殖生物学会の論文
著者
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Matsumoto Kazuya
Department of Reproductive and Developmental Biology, The Institute of Medical Science, The Universi
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Matsumoto Kazuya
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Saeki K
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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Saeki Kazuhiro
Department of Biological Sciences, Faculty of Science, Nara Women's University
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HOSOI Yoshihiko
Department of Genetic Engineering, Kinki University
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IRITANI Akira
Department of Genetic Engineering, Kinki University
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Hosoi Yoshihiko
Department Of Genetic Engineering Graduate School Of Biology Oriented Science And Technology Kinki U
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KASAMATSU Aya
Department of Genetic Engineering, Kinki University
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TAMARI Tomohiro
Department of Genetic Engineering, Kinki University
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IWAMOTO Daisaku
Department of Genetic Engineering, Kinki University
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TATEMIZO Atsuhiro
Department of Genetic Engineering, Kinki University
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Matsumoto Kazuya
Department Of Applied Chemistry Kanagawa Institute Of Technology
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Iritani Akira
Department of Animal Science, College of Agriculture, Kyoto University
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HOSOI Yoshihiko
Department of Biology-Oriented Science and Technology, Division of Genetic Engineering, Kinki University
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