Carbonic anhydrase II was detected in urine of a patient with osteosarcoma during high-dose methotrexate and leucovorin rescue therapy
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概要
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Using a 5-20% (linear gradient) polyacrylamide slab gel electrophoresis, we studied the urinary protein collected from a 14-year-old girl with osteosarcoma during high-dose methotrexate (MTX) and leucovorin rescue therapy. A distinct protein band of approximately 30 kDa (P-30 protein) was observed in addition to albumin. We have never before encountered a 30 kDa urinary protein. Therefore, we first studied the P-30 protein by peptide mass fingerprinting and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). We obtained the amino acid sequence “YDPSLKPLSVSYDQATSLR”, which is the same as that of amino acids (40-58) of human carbonic anhydrase II (CAII). Then, we confirmed this protein by Western blotting using anti-human CAII antibody. This enzyme distributes widely in the human bodies, in erythrocytes, kidney, and pancreas. We considered that this CAII originated from the kidney in association with high-dose MTX and leucovorin rescue therapy.
- 日本電気泳動学会の論文
- 2007-03-01
著者
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MORITA Tetsuro
Department of Clinical Nutrition, Kawasaki University of Medical Welfare
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HATANO Hiroshi
Department of Electrical and Electronic Engineering, Faculty of Science and Technology, Shizuoka Ins
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Ishikawa Naoko
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Morita Tetsuro
Department Of Orthopedics Niigata Cancer Center Hospital
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Morita Tetsuro
Department Of Clinical Nutrition Faculty Of Medical Professions Kawasaki University Of Medical Welfa
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UCHIYAMA TORU
Department of Pediatric Oncology, Institute of Development, Aging and Cancer, Tohoku University
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SATO Toyoji
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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KANEKO Takushi
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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TOYAMA Takako
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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ENOMOTO Shinichi
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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OHASHI Tatsuya
Biochemical Laboratory, Medical Development Center, Nitto Boseki Co., Ltd.
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SHIMA Yukio
Laboratory of Biochemistry, Kyorin University School of Health Sciences
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KIMURA Akira
Kitasato Junior College
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SEGAWA Hiroyuki
Department of Orthopedics, Niigata Cancer Center Hospital
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KOBAYASHI Hiroto
Department of Orthopedics, Niigata Cancer Center Hospital
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Haga Hiroko
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Sato Toyoji
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Shima Yukio
Laboratory Of Biochemistry Kyorin University School Of Health Sciences
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Toyama Takako
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Uchiyama Toru
Department Of Orthopedics Niigata Cancer Center Hospital
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Uchiyama Toru
Department Of Orthopedic Surgery Niigata University School Of Medicine
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Kaneko Takushi
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Ohashi Tatsuya
Biochemical Laboratory Nitto Boseki Co. Ltd.
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Hatano H
Yakult Central Institute For Microbiological Research
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Hatano Hiroshi
Optics Technology Division Takatsuki Laboratory Minolta Co. Ltd.
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Kobayashi Hiroto
Department Of Orthopedics Niigata Cancer Center Hospital
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Kobayashi Hiroto
Department Of Orthopedic Surgery Niigata Cancer Center Hospital
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Segawa H
Department Of Orthopedics Niigata Cancer Center Hospital
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Segawa Hiroyuki
Department Of Orthopaedic Surgery Niigata University
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Enomoto Shinichi
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Hatano Hiroshi
Department Of Applied Physics Osaka University
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Morita Tetsuro
Department Of Orthopedic Surgery Niigata Cancer Center Hospital
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Kobayashi Hiroto
Department Of Orthopeadic Surgery Tokyo Medical University
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KOBAYASHI Hiroto
Department of Applied Physics, Graduate School of Engineering, The University of Tokyo
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SHIMA Yukio
Laboratory of Analytical Chemistry and Biochemistry, Department of Medical Technology, Kyorin University Faculty of Health Sciences
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