<Original Paper>A Collection Method and Its Application to Biliopancreatic Exocretional Experiments on Conscious Rats
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概要
- 論文の詳細を見る
A biliopancreatic exocrine apparatus was attached to a conscious rat without housing it in a Bollman type cage. The rat was allowed exercise and free access to solid food and water in an individual stainless steel cage in a room lit automatically from 08:00 to 20:00hr. The rat survived for several days with this apparatus and it was possible to collect biliopancreatic juice at any time. The apparatus was constructed using a biliopancreatic silastic fistula, a gastric cannula, a dorsal cover made from a disposable syringe and tubing available at any physiological laboratory. To test the viability of the apparatus, the rat's pancreas was indirectly stimulated byintroducing various nutrients via the gastric tube. In multiple tests, the same nutrient elicited a consistent response from the pancreas but casein stimulated more strongly than starch. This system using rats can be useful for several other experiments such as observing the pancreatic exocrine response to other stimulants. Moreover, it is useful due to low cost and because the technique is simple, many rats can be prepared simultaneously.
- 川崎医療福祉大学の論文
著者
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MORITA Tetsuro
Department of Clinical Nutrition, Kawasaki University of Medical Welfare
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Morita Tetsuro
Department Of Clinical Nutrition Faculty Of Medical Professions Kawasaki University Of Medical Welfa
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Ono Akifumi
Department Of Clinical Nutrition Faculty Of Medical Professions Kawasaki University Of Medical Welfa
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Ono Akifumi
Department Of Clinical Nutrition Faculty Of Health Science And Technology Kawasaki University Of Med
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Matsueda Shuji
Department of Clinical Nutrition, Faculty of Health Science and Technology, Kawasaki University of M
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Matsueda Shuji
Department Of Clinical Nutrition Faculty Of Health Science And Technology Kawasaki University Of Med
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Ono Hisami
Department of Nursing, Niimi Women's College
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Matsumoto Yoshinobu
Department of Clinical Nutrition, Faculty of Medical Professions, Kawasaki University of Medical Wel
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TAKEMASA Mutsuko
Department of Clinical Nutrition, Faculty of Medical Professions, Kawasaki University of Medical Wel
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Ono Akifumi
Department Of Clinical Nutrition Faculty Of Medical Professions Kawasaki University Of Medical Welfa
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Matsueda Shuji
Department Of Clinical Nutrition Faculty Of Health Science And Technology Kawasaki University Of Med
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Takemasa Mutsuko
Department Of Clinical Nutrition Faculty Of Medical Professions Kawasaki University Of Medical Welfa
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Matsumoto Yoshinobu
Department Of Clinical Nutrition Faculty Of Medical Professions Kawasaki University Of Medical Welfa
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Ono Hisami
Department Of Nursing Niimi Women's College
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