Characterization of serum alkaline phosphatase separated at or near the origin in polyacrylamide gel using detergents, antibodies and neuraminidase
スポンサーリンク
概要
- 論文の詳細を見る
We studied serum samples which showed alkaline phosphatase (ALP) activity at or near the origin of the polyacrylamide gel electrophoresis. 1) Sucrose monolaurate (final concentration, 1.7%) treatment produced a new ALP band (SM-ALP band). 2) The SM-ALP band disappeared from the original region with anti-human placental ALP antibody and appeared at the high molecular region. 3) ALP activity of SM-ALP band was completely inactivated by heat treatment (at 65°C for 10 min). 4) The molecular mass was estimated to be approximately 350 kDa by a 5-20% (linear gradient) polyacrylamide slab gel electrophoresis. We concluded that the SM-ALP band was intestinal ALP tetramer.
- 2008-03-01
著者
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SATO Toyoji
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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OHASHI Tatsuya
Biochemical Laboratory, Medical Development Center, Nitto Boseki Co., Ltd.
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SHIMA Yukio
Laboratory of Biochemistry, Kyorin University School of Health Sciences
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Miura Toshihide
Biochemical Laboratory, Nitto Boseki Co., Ltd.
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Haga Hiroko
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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HIRANO Yuji
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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KOBAYASHI Akiko
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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HARUKI Akira
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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HARA Mitsumasa
Division of Clinical Chemistry, Niigata Cancer Center Hospital
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SASAGAWA Kumiko
Biochemical Laboratory, Nitto Boseki Co., Ltd.
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WATANABE Shinichiro
Internal Medicine, Minami Senju Hospital
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Sasagawa Kumiko
Biochemical Laboratory Nitto Boseki Co. Ltd.
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Sato Toyoji
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Shima Yukio
Laboratory Of Biochemistry Kyorin University School Of Health Sciences
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Hatano Hiroshi
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Hirano Yuji
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Haruki Akira
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Hara Mitsumasa
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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Ohashi Tatsuya
Biochemical Laboratory Nitto Boseki Co. Ltd.
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Hatano H
Yakult Central Institute For Microbiological Research
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Hatano Hiroshi
Optics Technology Division Takatsuki Laboratory Minolta Co. Ltd.
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Miura Toshihide
Biochemical Laboratory Nitto Boseki Co. Ltd.
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Watanabe Shinichiro
Internal Medicine Minami Senju Hospital
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Kobayashi Akiko
Division Of Clinical Chemistry Niigata Cancer Center Hospital
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SHIMA Yukio
Laboratory of Analytical Chemistry and Biochemistry, Department of Medical Technology, Kyorin University Faculty of Health Sciences
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