実験動物学:ラット一般毒性試験におけるジエチルエーテル麻酔の代替法としてのイソフルラン希釈液を用いた麻酔法
スポンサーリンク
概要
- 論文の詳細を見る
Despite its explosive properties and toxicity to both animals and humans, diethyl ether is an agent long used in Japan in the anaesthesia jar method of rat anaesthetises. However, in response to a recent report from the Science Council of Japan condemning diethyl ether as acceptable practice, we searched for an alternative rat anaesthesia method that provided data continuous with pre-existing regular toxicology studies already conducted under diethyl ether anaesthesia. For this, we examined two candidates; 30% isoflurane diluted with propylene glycol and pentobarbitone. Whereas isoflurane is considered to be one of the representatives of modern volatile anaesthetics, the method of propylene glycol-diluted 30% isoflurane used in this study was our modification of a recently reported method revealed to have several advantages as an inhalation anaesthesia. Intraperitoneal pentobarbitone has long been accepted as a humane method in laboratory animal anaesthesiology. These 2 modalities were scrutinized in terms of consistency of haematology and blood chemistry with previous results using ether. We found that pentobarbitone required a much longer induction time than diethyl ether, which is suspected to be the cause of fluctuations in several haematological and blood chemical results. Conversely, only calcium ion concentration showed a slight difference from traditional results in the case of 30% isoflurane. Additionally, serum prolactin and corticosterone levels indicated that 30% isoflurane induced less stress than ether, confirming that 30% isoflurane can both provide results consistent with diethyl ether, while at the same time remove its disadvantages. As such 30% isoflurane appears to be a strong alternative anaesthetic agent for future regular toxicology studies in Japan.
- 社団法人 日本獣医学会の論文
- 2007-11-25
著者
-
Tamura Toru
キッセイ薬品工業中央研究所
-
Tamura Toru
Toxicology Research Laboratory R & D Kissei Pharmaceutical Co. Ltd.
-
Tamura Toru
信州大学 大学院総合工学系研究科
-
KURODA Junji
Toxicology Laboratory, Kissei Pharmaceutical Co., Ltd.
-
Kagami Hiroshi
Fac. Of Agriculture Shinshu Univ. Jpn
-
MUTO Shin-ichi
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co. Ltd.
-
Muto Shin-ichi
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Kusama Hiroshi
Department Of Development Research Kissei Pharmaceutical Co. Ltd.
-
Kuroda Junji
Toxicology Lab. Kissei Pharmaceutical Co. Ltd.
-
Kuroda J
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Kuroda Junji
Toxicology
-
TAKASHIMA Kayoko
Toxicogenomics Project, National Institute of Biomedical Innovation
-
NAGATE Toshiaki
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
CHINO Tomonobu
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
NISHIYAMA Chizuru
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
OKUHARA Daisuke
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
TAHARA Toru
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
MARUYAMA Yoshimasa
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
KASAHARA Hiroko
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
KOBAYASHI Sayaka
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
MOTOKAWA Yoshiyuki
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd.
-
Kasahara Hiroko
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Chino Tomonobu
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Nagate Toshiaki
Toxicology Research Laboratory R & D Kissei Pharmaceutical Co. Ltd.
-
Okuhara Daisuke
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Kobayashi Sayaka
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Nagate Toshiaki
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Motokawa Yoshiyuki
Toxicology Research Laboratory R&d Kissei Pharmaceutical Co. Ltd.
-
Takashima Kayoko
Toxicogenomics Project National Institute Of Health Sciences
関連論文
- ラット一般毒性試験におけるジエチルエーテル麻酔の代替法としてのイソフルラン希釈液を用いた麻酔法(実験動物学)
- Comparison of enhancing effects of different goitrogen treatments in combination with β-estradiol-3-benzoate for establishing a rat two-stage thyroid carcinogenesis model to detect modifying effects of estrogenic compounds
- Lack of Modifying Effects of Combined Treatment of t-butylhydroquinone and Sodium Nitrite on Forestomach Carcinogenesis in rasH2 Mice Initiated with N-ethyl-N-nitrosourea
- Tumorigenic Susceptibility of Catechol on the Gastric Mucosa in rasH2 Mice
- Lack of Modifying Effect of Glycerol in Pulmonary Carcinogenesis in rasH2 Mice Induced by Urethane or 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
- Modulatory effects of hepatic, renal or thyroidal impairments on amitrole-induced thyroid carcinogenesis in rats pretreated with DHPN (CARCINOGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Safety evaluetion of Bt protein by dietary administration to a gastro-intestinal impairment rat model (GENERAL TOXICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Modifying Effects of 2,6-Dimethylaniline on Nasal Carcinogenesis in RasH2 Mice Initiated with 4-Methylnitrosamino)-1-(3-pyridyl)-1-butanone
- Lack of Modifying Effects of 2,6-Dimethylaniline on Lung Carcinogenesis in Transgenic Mice Carrying Human Prototype c-Ha-ras Gene Initiated with 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone
- Difference in the Types of Uterine Tumors between Heterozygous p53-deficient and Wild Type CBA Mice Treated with Ethinylestradiol after N-ethyl-N-nitrosourea Initiation