Characterization of Direct Immobilized Probe DNA on Partially Functionalized Diamond Solution-Gate Field-Effect Transistors
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概要
- 論文の詳細を見る
Amino groups were functionalized directly on the diamond surface after treating 0.5 monolayer of oxidation for detection of DNAs. Also, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. Specific hybridization with 21-mer DNA at a concentration of 100 nM could be clearly detected by two methods, fluorescence microscopy and diamond solution-gate field-effect transistors (SGFETs). DNA hybridization was confirmed using Cy-5-labeled target DNA on a micropatterned diamond surface. The changes in gate potential by the negative charge of immobilized or hybridized DNA were measured on SGFETs and hybridization efficiency on the functionalized diamond surface was estimated as about 40%.
- Japan Society of Applied Physicsの論文
- 2006-11-25
著者
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KAWARADA Hiroshi
Department of Electrical Engineering, Faculty of Engineering, Osaka University
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Yang Jung-hoon
School Of Science And Engineering Waseda University
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Song Kwang-soup
School Of Science And Engineering Waseda University
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Yang Jung-Hoon
Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda Uni
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Song Kwang-Soup
Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda Uni
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Kuga Shouma
Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda Uni
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Kuga Shouma
School Of Science And Engineering Waseda University
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