Cloning and Comparison of Third β-Glucoside Utilization (bglEFIA) Operon with Two Operons of Pectobacterium carotovorum subsp. carotovorum LY34
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概要
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A third bgl operon containing bglE, bglF, bglI, and bglA was isolated from Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34). The sequences of BglE, BglF, and Bgll were similar to those of the phosphotransferase system (PTS) components IIB, IIC, and IIA respectively. BglF contains important residues for the phosphotransferase system. The amino acid sequence of BglA showed high similarity to various 6-phospho-β-glucosidases and to a member of glycosyl hydrolase family 1. Sequence and structural analysis also revealed that these four genes were organized in a putative operon that differed from two operons previously isolated from Pcc LY34, bglTPB (accession no. AY542524) and ascGFB (accession no. AY622309). The transcription regulator for this operon was not found, and the EII complexes for PTS were encoded separately by three genes (bglE, bglF, and bglI). The BglA enzyme had a molecular weight estimated to be 57,350 Da by SDS–PAGE. The purified β-glucosidase hydrolyzed salicin, arbutin, ρNPG, ρNPβG6P, and MUG, exhibited maximal activity at pH 7.0 and 40 °C, and displayed enhanced activity in the presence of Mg2+ and Ca2+. Two glutamate residues (Glu178 and Glu378) were found to be essential for enzyme activity.
- 社団法人 日本農芸化学会の論文
- 2006-04-23
著者
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Kim Yong
Division Of Gynecologic Oncology Department Of Obstetrics And Gynecology Yonsei University College O
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Cho Soo
Division Of Applied Life Science Gyeongsang National University
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Hong Su
Division Of Applied Life Science Gyeongsang National University
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Lim Yong
Department of Horticulture, Chungnam National University
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Kim Min
Division Of Applied Life Science Gyeongsang National University
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Lee Sun
Division Of Physical Metrology Korea Research Institute Of Standards And Science
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Lim Yong
Department Of Horticulture Chungnam National University
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AN Chang
Division of Applied Life Science, Gyeongsang National University
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CHO Kye
Division of Applied Life Science, Gyeongsang National University
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KIM Hoon
Department of Agricultural Chemistry, Sunchon National University
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YUN Han
Division of Applied Life Science, Gyeongsang National University
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Cho Kye
Dep. Of Food Sci. Jinju National Univ.
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Kim Yong
Division Of Allergy And Respiratory Medicine Department Of Internal Medicine Genome Research Center
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Yun Handae
Division Of Applied Life Science Gyeongsang National University
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Cho S
Division Of Applied Life Science Gyeongsang National University
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An Chang
Division Of Applied Life Science Gyeongsang National University
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Lee Sun
Division Of Cardiology Department Of Internal Medicine Chonbuk National University Medical School
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Kim Yong
Division Of Applied Life Science Gyeongsang National University
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Lim Yong
Department Of Cellular-molecular Medicine School Of Medicine Chosun University
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Lee Sun
Division Of Applied Life Science Gyeongsang National University
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Kim Hoon
Department Of Agricultural Chemistry Sunchon National University
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Yun Han
Division Of Applied Life Science (bk21 Program) Gyeongsang National University
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