Analysis of bgl Operon Structure and Characterization of β-Glucosidase from Pectobacterium carotovorum subsp. carotovorum LY34
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概要
- 論文の詳細を見る
A putative bgl operon of Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34) was isolated. Sequence analysis of the 5,557 bp cloned DNA fragment (accession no. AY542524) showed three open reading frames (bglT, bglP, and bglB) predicted to encode 287, 633, and 468 amino acid proteins respectively. BglT and BglP ORFs show high similarity to that of the Pectobacterium chrysanthemi ArbG antiterminator and ArbF permease respectively. Also, the latter contains most residues important for phosphotransferase activity. The amino acid sequence of BglB showed high similarity to various β-glucosidases and is a member of glycosyl hydrolase family 1. The purified BglB enzyme hydrolyzed salicin, arbutin, pNPG, and MUG. The molecular weight of the enzyme was estimated to be 53,000 Da by SDS–PAGE. The purified β-glucosidase exhibited maximal activity at pH 7.0 and 40 °C, and its activity was enhanced in the presence of Mg2+. Two glutamate residues (Glu173 and Glu362) were found to be essential for enzyme activity.
- 社団法人 日本農芸化学会の論文
- 2004-11-23
著者
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Park Sang
National Research Institute For Earth Science And Disaster Prevention (nied)
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Lim Woo
Division Of Applied Life Science Gyeongsang National University
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Hong Su
Division Of Applied Life Science Gyeongsang National University
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Lim Yong
Department of Horticulture, Chungnam National University
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Kim Min
Division Of Applied Life Science Gyeongsang National University
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Lee Jong
National Institute Of Safety Research Seoul Korea
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Kim Eun
Division Of Applied Life Science Gyeongsang National University
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Shin Eun
Division Of Applied Life Science Gyeongsang National University
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Lim Yong
Department Of Horticulture Chungnam National University
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AN Chang
Division of Applied Life Science, Gyeongsang National University
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YUN Han
Division of Applied Life Science, Gyeongsang National University
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WOO Jong
National Horticultural Research Institute, RDA
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Woo Jong
National Horticultural Research Institute Rda
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Park Sangryeol
National Institute Of Agricultural Biotechnology Rda
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Yun Handae
Division Of Applied Life Science Gyeongsang National University
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An Chang
Division Of Applied Life Science Gyeongsang National University
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Lim Yong
Department Of Cellular-molecular Medicine School Of Medicine Chosun University
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Yun Han
Division Of Applied Life Science (bk21 Program) Gyeongsang National University
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Lee Jong
National Institute Of Agricultural Biotechnology Rda
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Lee Jong
National Center for Nanoprocess and Equipments, Korea Institute of Industrial Technology, Gwangju 500-480, Korea
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PARK Sang
National Institute of Agricultural Biotechnology
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WOO Jong
National Horticultural Research Institute
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Lee Jong
National Center for Nanoprocess and Equipment, Korea Institute of Industrial Technology, Gwangju 500-480, Republic of Korea
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