An improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea
スポンサーリンク
概要
- 論文の詳細を見る
An efficient Agrobacterium-mediated genetic transformation method was established for Brassica juncea by investigating several factors responsible for successful gene transfer. Four-day-old cotyledon explants from in vitro grown seedlings were co-cultivated with Agrobacterium strain GV3101 harboring the binary vector EnPCAMBIA1302-YCF1, which contained the hygromycin phosphotransferase (HPT) gene as a selectable marker and the yeast cadmium factor 1 (YCF1) gene. Two days co-cultivation period on shoot induction medium (MS medium supplemented with 0.1 mg l−1 α-naphthaleneacetic acid, 1.0 mg l−1 6-benzyladenine, and 2.0 mg l−1 silver nitrate) containing 20 mg l−1 acetosyringone and five days delaying exposure of explants to selective agent enhanced transformation efficiency significantly. A three-step selection strategy was developed to select hygromycin resistant shoots. Hygromycin-resistant shoots were subsequently rooted on root induction medium. Rooted plantlets were transferred to pot-soil, hardened, and grown in a greenhouse until maturity. Using the optimized transformation procedure, transformation efficiency reached at 16.2% in this study. Southern blot analysis was performed to confirm that transgenes (HPT and YCF1) were stably integrated into the plant genome. All transgenic plants showed single-copy of transgene integration in the host genome. Segregation analysis of T1 progeny showed that the transgenes were stably integrated and transmitted to the progeny in a Mendelian fashion.
- 2011-03-25
著者
-
Sultana Sayeda
Department of Biosciences, Chungnam National University
-
Lee Youngsook
POSTECH-UZH Cooperative Laboratory, Department of Integrative Bioscience and Biotechnology, Pohang U
-
Choi Kwan
Department Of Life Science Pohang University Of Science And Technology
-
Lim Yong
Department Of Horticulture Chungnam National University
-
Liu Jang
Plant And Animal Cell Technology Research Division Korea Research Institute Of Bioscience
-
Lim Yong
Department Of Cellular-molecular Medicine School Of Medicine Chosun University
-
Min Sung
Plant Systems Engineering Research Center Korea Research Institute Of Bioscience And Biotechnology (
-
Song Won
Postech-uzh Cooperative Laboratory Department Of Integrative Bioscience And Biotechnology Pohang Uni
-
Jeong Won
Plant Systems Engineering Research Center Korea Research Institute Of Bioscience And Biotechnology (
-
Bhuiyan Mohammed
Department Of Agricultural Biology Chungnam National University
-
Lee Youngsook
Postech-uzh Cooperative Laboratory Department Of Integrative Bioscience And Biotechnology Pohang Uni
-
Liu Jang
Plant Systems Engineering Research Center Korea Research Institute Of Bioscience And Biotechnology (
-
Sultana Sayeda
Department Of Biosciences Chungnam National University
-
Choi Kwan
Department Of Agricultural Biology Chungnam National University
-
Min Sung
Plant Genome Research Center Korea Research Institute Of Bioscience And Biotechnology (kribb)
-
Min Sung
Plant Cell And Molecular Biology Research Unit Korea Research Institute Of Bioscience And Biotechnol
関連論文
- Epithelial-mesenchymal transition gene signature to predict clinical outcome of hepatocellular carcinoma
- An improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea
- Degradation of Phytochrome Interacting Factor 3 in Phytochrome-Mediated Light Signaling
- Antibiotic Activities of Synthetic All-D Peptide against the Clinically Isolated Drug-Resistant and Biofilm-Forming Strains
- Structural Insights of the Nucleotide-Dependent Conformational Changes of Thermotoga maritima MutL Using Small-Angle X-ray Scattering Analysis
- Cloning and Comparison of Third β-Glucoside Utilization (bglEFIA) Operon with Two Operons of Pectobacterium carotovorum subsp. carotovorum LY34
- Analysis of bgl Operon Structure and Characterization of β-Glucosidase from Pectobacterium carotovorum subsp. carotovorum LY34
- Fast Determination of the Ripeness Stage of Strawberries Using Infrared Spectroscopy Combined with Principal Component Analysis
- In Vitro Flowering of Ginseng (Panax ginseng C. A. Meyer) Zygotic Embryos Induced by Growth Regulators
- Synthesis and Properties of Cyclic Peptides Arranging a Pair of Porphyrin and Fullerene C_
- Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.)
- Development of Two Stage Culture Process by Optimization of Inorganic Salts for Improving Catharanthine Production in Hairy Root Cultures of Catharanthus roseus
- Inhibitory Effects of Docetaxel on Platelet-Derived Growth Factor (PDGF)-BB–Induced Proliferation of Vascular Smooth Muscle Cells Through Blocking PDGF-Receptor β Phosphorylation
- Proteomic analysis of Korean ginseng (Panax ginseng C. A. Meyer)
- Presence of Multiple cDNAs Encoding an Isoform of ADP-Glucose Pyrophosphorylase Large Subunit from Sweet Potato and Characterization of Expression Levels
- Production and Secretion of Indole Alkaloids in Hairy Root Culrures of Catharanthus roseus : Effects of In Situ Adsorption, Fungal Elicitation and Permeabilization
- Procedure for Biomass Estimation Considering the Change in Biomass Volume during High Density Culture of Hairy Roots
- Enhanced Production of Shikonin by Lithospermum erythrorhizon Cells Immobilized in Polyurethane Foam Matrices
- Development and linkage mapping of unigene-derived microsatellite markers in Brassica rapa L.
- An improved method for Agrobacterium-mediated genetic transformation from cotyledon explants of Brassica juncea
- Selection and Characterizations of Radiation-induced Salinity-tolerant Lines in Rice
- Inhibitory Effects of Docetaxel on Platelet-Derived Growth Factor (PDGF)-BB-Induced Proliferation of Vascular Smooth Muscle Cells Through Blocking PDGF-Receptor β Phosphorylation
- Clitocybin A, a Novel Isoindolinone, From the Mushroom Clitocybe aurantiaca, Inhibits Cell Proliferation Through G1 Phase Arrest by Regulating the PI3K/Akt Cascade in Vascular Smooth Muscle Cells