Antigenicity of Cell Wall Mannans of Candida albicans NIH A-207 Strain Cells Cultured in Galactose-Added Yeast Nitrogen Base Medium(Miscellaneous)
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概要
- 論文の詳細を見る
The cultivations of the Candida albicans NIH A-207 strain (A-strain) for 5 d at 27 and 37℃ in 500mм galactose-added yeast nitrogen base medium (YNB-Gal) decreased the growth of blastoconidia and the pH in the cultures, with dry weights of 56 and 47% and with pHs of 2.41 and 2.47, compared with the dry weight of 100% and pH of 5.63 for a standard cultivation of 2 d at 27℃ in the yeast extract-added Sabouraud liquid medium (YSLM). The cells obtained by cultivations at 27 and 37℃ in the YNB-Gal clearly decreased the agglutination against serum factors 4, 5, and 6 in the commercially available kit 'Candida Check', especially at 37℃, in contrast to those obtained by the standard cultivation. It was also revealed by ^1H-NMR analysis that both the mannans obtained from cultures at 27 and 37℃ in the YNB-Gal had drastically lost a phosphate group and a β-1,2-linked mannopyranose unit, and increased the non-reducing terminal α-1,3-linked mannopyranose unit, especially at 37℃.
- 2005-02-01
著者
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OKAWA Yoshio
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy
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Miyauchi Masayoshi
Department Of Infection And Host Defense Tohoku Pharmaceutical University
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Okawa Y
Department Of Infection And Host Defense Tohoku Pharmaceutical University
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Okawa Y
Second Department Of Hygienic Chemistry Tohoku College Of Pharmacy
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GIUMMELLY Philippe
Department of Infection and Host Defense, Tohoku Pharmaceutical University
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MIYAUCHI Masayoshi
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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GOTO Kouji
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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GIUMMELLY Philippe
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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Okawa Yoshio
Second Dep. Of Hygienic Chemistry Tohoku Pharmaceutical Univ.
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Goto Kouji
Second Department Of Hygienic Chemistry Tohoku Pharmaceutical University
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Giummelly Philippe
Department Of Infection And Host Defense Tohoku Pharmaceutical University
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