A Novel Protein with Alkaline Phosphatase and Protease Inhibitor Activities in Streptomyces hiroshimensis
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概要
- 論文の詳細を見る
A novel alkaline phosphatase (S-ALP) was found in the culture filtrate of Streptomyces hiroshimensis IFO 12785. Purification was achieved on Sephadex G-75 column, palmitoylated gauze column, and Superdex 75 HR column chromatographies. The molecular weight of S-ALP was estimated to be 14200 by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). The isoelectric point is 9.2. S-ALP had maximum enzyme activity at pH 9.5. S-ALP efficiently catalyzed both p-nitrophenyl phosphate and p-nitrophenyl phosphorylcholine substrates, particularly the latter. The N-terminal amino acid sequence (25 residues) of S-ALP was 60 to 72% identical' to that of Streptomyces subtilisin inhibitor-like proteins. S-ALP exhibited trypsin inhibition in addition to a strong inhibition of subtilisin.
- 公益社団法人日本薬学会の論文
- 2002-07-01
著者
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Goto M
Toin University Of Yokohama Faculty Of Medical Engineering Department Of Clinical Engineering Divisi
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Okawa Yoshio
Dep. Of Infection And Host Defense Tohoku Pharmaceutical Univ.
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Okawa Yoshio
Second Department Of Hygienic Chemistry Tohoku Pharmaceutical University
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Okawa Yoshio
Second Dep. Of Hygienic Chemistry Tohoku Pharmaceutical Univ.
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Shibuya Naomi
Second Department Of Hygienic Chemistry Tohoku Pharmaceutical University
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NITTA Masayuki
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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GOTO Mariko
Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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Nitta Masayuki
Second Department Of Hygienic Chemistry Tohoku Pharmaceutical University
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