Structural Changes of Cell Wall Mannans of Candida guilliermondii IFO 10279 Strain Cells Cultured at High Temperature(Miscellaneous)

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The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34℃ for 48h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27℃ and 33℃ and then at 27℃ (33-27℃). This strain showed little growth at higher than 35℃. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34℃, and only the cells at 34℃ revealed a failure of cytokinesis. The structure of the mannans revealed by ^1H-NMR analysis that the mannans obtained at both 33 and 34℃ had drastically decreased two consecutive β-1, 2-linked mannopyranose units at the nonreducing terminal of the α-linked oligosaccharides and increased one β-1, 2-linked mannopyranose unit at the nonreducing terminal attached to the α-1, 3-linked mannose unit and the non-reducing terminal α-1, 3- and α-1, 2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34℃ had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit 'Candida Check'.
2006-02-01