umu test 自動分析装置の構築と性能評価

概要

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The new method for umu test using the automatic detection device is constructed as a simple method to monitor the safety of the environmental water. This device applies the CFA (Continuous Flow Analysis) method using AutoAnalyzer II (Bran+Luebbe). After addition of samples and phosphate-buffer, the culture is incubated for 1.5 hours in the teflon tube wound in a coil in the 36°C water bath. Then β-galactosidase enzymatic reaction is acted in the teflon-tube coil. The absorbance is measured at 600nm and 420nm in the spectrophotometers with a flow cell which are controlled by Visual Basic program. AF-2, 4-NQO and 2-AA which are major mutagens were measured by both this new method and the usual manual method of umu test. The automatic detection device was more sensitive than usual method and had higher accuracy (CV = 5.5-12.1%) than usual method (CV = 11.1-14.9%). This result suggests that this automatic detection device can be used as the monitoring methods.
社団法人　日本水環境学会の論文
1999-10-10