Characteristics of Mixed Reverse Micelles from Ternary Surfactant with Di(2-Ethylhexyl) Tails
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概要
- 論文の詳細を見る
To design a novel reverse micellar system for protein extraction, binary and ternary reverse micellar systems composed of sodium bis(2-ethylhexyl) sulfosuccinate (AOT), di(2-ethylhexyl)phosphoric acid (DEHPA) and di(2-ethylhexyl) phosphorothioic acid (DEPTA) in isooctane are examined for the water solubilization and the extraction characteristics of proteins. The AOT-DEHPA-DEPTA ternary surfactant system is found to be suitable for protein extraction. The size of the mixed reverse micelle was determined by the small angle X-ray scattering method. It appears that both the water content and the micelle size for the ternary system are larger than those for the AOT and DEPTA single systems. Hemoglobin was successfully extracted by the ternary system, and back-extraction can be realized by use of a weaker alkaline solution (pH 9.5) with the addition of a small amount of n-octanol in the organic phase.
- 社団法人 化学工学会の論文
著者
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Takeuchi Hiroshi
Department Of Psychiatry Nagoya City University Medical School
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YAMANE Takashi
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya Univer
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Yamane T
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Rong L
Nagoya Univ. Nagoya Jpn
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RONG Long
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya Univer
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Takeuchi Hiroshi
Department Of Chemical Engineering Nagoya University
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Takeuchi Hiroshi
Laboratory Of Molecular And Cellular Biochemistry Faculty Of Dental Science And Station For Collabor
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Yamane Takashi
Department Of Biotechnology And Biomaterial Chemistry Graduate School Of Engineering Nagoya Universi
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Takeuchi Hiroshi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Yamane Takashi
Department of Applied Chemistry and Department of Synthetic Chemistry, Faculty of Engineering, Nagoya University
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