Fractionation of IgG Fragments Using Reversed Micellar Extraction
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概要
- 論文の詳細を見る
Reversed micellar extraction was applied for the fractionation of IgG fragments. With isooctane solution containing 50mM AOT, Fab, Fc and IgG were extracted to the micellar phase. Each protein had an optimum pH range in the extraction. Fab and Fc were separated from the mixture at pH 7.0,with Fab being extracted to the micellar phase and Fc remaining in the aqueous phase. Extracted Fab fragments were recovered by brining them into contact with 6M guanidine/HCI followed by dilution with PBS. Fab and Fc fragments were separated and recovered by reversed micellar extraction from IgG lysate digested with papain. Since the procedure is simple and rapid compared with column chromatography, mass preparation of the fragments can be expected by this method.
- 公益社団法人日本生物工学会の論文
- 1994-01-25
著者
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Takeuchi Hiroshi
Department Of Electronic Engineering Faculty Of Technology Tokyo University Of Agriculture And Techn
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Kamihira Masamichi
(present Address)department Of Biotechnology.
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Takahashi Katsuroku
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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YANAGISAWA HIDETOSHI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Yanagisawa Hidetoshi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Takeuchi Hiroshi
Department Of Chemical Engineering Nagoya University
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Takeuchi H
Nagoya Univ. Nagoya Jpn
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Takeuchi Hiroshi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Takahashi Katsuroku
Departmennt of Chemical Engineering, Nagoya University
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