A REVERSED MICELLAR SYSTEM USING MIXED SURFACTANTS OF SODIUM BIS (2-ETHYLHEXYL) SULFOSUCCINATE AND DI(2-ETHYLHEXYL) PHOSPHORIC ACID FOR EXTRACTION OF PROTEINS
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概要
- 論文の詳細を見る
Protein extraction, including sollubilization of water, in a reversed micellar solution has been studied using mixed surfactants of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and di(2-ethylhexyl)phosphoric acid (DEHPA) in isooctane . It appears that the mixed micelles could be formed from AOT and charged species of DEHPA molecules in the organic phase, while uncharged DEHPA does not take part in forming the micelles. The solubilization of water into the mixed micelles depends on pH and salt concentration in the aqueous solution as well as composition and concentration of surfactants. Hemoglobin having relatively large molecular weight could be successfully extracted by the mixed micelles. Furthermore, it was found that the addition of n-hexanol in the micellar solution is useful for stripping of proteins from the micelles into dilute alkaline solution.
- 1994-10-20
著者
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Watanabe K
Department Of Industrial Chemistry Niihama National College Of Technology
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Kinugasa T
Niihama National Coll. Technol. Niihama Jpn
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KINUGASA TAKUMI
Depertment of Industrial Chemisty, Niihama National College of Technology
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HISAMATSU ATSUKO
Depertment of Industrial Chemisty, Niihama National College of Technology
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WATANABE KUNIO
Depertment of Industrial Chemisty, Niihama National College of Technology
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TAKEUCHI HIROSHI
Depertment of Chemical Engineering, Nagoya University
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渡部 和広
Division Of Materials Science And Engineering Graduate School Of Engineering Hokkaido University
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Hisamatsu Atsuko
Depertment Of Industrial Chemisty Niihama National College Of Technology
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Takeuchi Hiroshi
Department Of Chemical Engineering Nagoya University
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Takeuchi Hiroshi
Laboratory Of Molecular And Cellular Biochemistry Faculty Of Dental Science And Station For Collabor
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