APPLICATION OF PLURONIC F68 FOR AQUEOUS TWO-PHASE EXTRACTION OF PROTEINS

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概要

• 論文の詳細を見る

• Studies were made of the application of a block copolymer of polyethylene oxide and polypropylene oxide, Pluronic F68, to aqueous two-phase extraction of proteins. For Pluronic F68/salt systems, the effects of temperature, salt concentration and pH on the binodials and protein partitioning were studied. Pluronic F68/salt system had a region in which Pluronic F68 becomes solid in phase diagram at 25°C. Partitioning of the proteins between the two phases could be controlled by varying salt composition. Further applicability of the Pluronic F68/ammonium sulfate system was examined for the purification of monoclonal antibodies from various hybridoma culture broths. Aqueous two-phase extraction provided a simple and rapid separation and concentration method with high recovery.

• 社団法人 化学工学会の論文
• 1993-04-20

著者

• Takeuchi Hiroshi

  Department Of Electronic Engineering Faculty Of Technology Tokyo University Of Agriculture And Techn

• Kitahara Toshihiko

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• KAMIHARA Masamichi

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Kamihara Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Takeuchi Hiroshi

  Department Of Chemical Engineering Nagoya University

• Takeuchi Hiroshi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

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