Electrochemical Characterization of O2 Plasma Functionalized Multi-Walled Carbon Nanotube Electrode for Legionella pneumophila DNA Sensor
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概要
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An electrochemical DNA sensor for Legionella pneumophila detection was constructed using O2 plasma functionalized multi-walled carbon nanotube (MWCNT) film as a working electrode (WE). The cyclic voltammetry (CV) results revealed that the electrocatalytic activity of plasma functionalized MWCNT (pf-MWCNT) significantly changed depending on O2 plasma treatment time due to some oxygen containing functional groups on the pf-MWCNT surface. Scanning electron microscope (SEM) images and X-ray photoelectron spectroscopy (XPS) spectra were also presented the changes of their surface morphologies and oxygen composition before and after plasma treatment. From a comparison study, it was found that the pf-MWCNT WEs had higher electrocatalytic activity and more capability of probe DNA immobilization: therefore, electrochemical signal changes by probe DNA immobilization and hybridization on pf-MWCNT WEs were larger than on Au WEs. The pf-MWCNT based DNA sensor was able to detect a concentration range of 10 pM–100 nM of target DNA to detect L. pneumophila.
- 2010-08-25
著者
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Park Eun
Department Of Anatomy And Cell Biology College Of Veterinary Medicine And School Of Agricultural Bio
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Kim Sun
School Of Life Sciences And Biotechnology Korea University:disease Model Research Center Biological
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Kim Jun
Department Of Bioengineering University Of California Los Angeles
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Min Nam
Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Korea
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Lee Jun-Yong
Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Korea
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Lee Cheol
School of Electrical Engineering, Korea University, Seoul 136-701, Korea
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Kim Jun
Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Korea
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KIM Jun
Department of Advanced Material Science and Engineering, Yonsei University
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Kim Sun
School of Electrical Engineering, Korea University, Seoul 136-701, Korea
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