Liquid Phase Immunoassay Using Magnetic Markers and Superconducting Quantum Interference Device
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概要
- 論文の詳細を見る
A liquid phase immunoassay utilizing magnetic markers and a high-$T_{\text{c}}$ superconducting quantum interference device (SQUID) was studied. In this method, the biological target is detected using magnetic markers, i.e., the magnetic signal from the markers that bound to the target is detected with the SQUID. The detection was performed in a solution containing both the bound and unbound (free) markers without using the so-called bound/free (BF) separation process. The bound markers were distinguished from the free markers by utilizing the Brownian rotation of the free markers. First, the properties of the free markers in the solution, such as the $M$–$H$ curve and magnetic relaxation, were measured to study the background signal from the free markers. Markers that exhibit remanence were used for the experiment. Using the obtained results, we discuss the effects of the residual earth field and aggregation of the markers on the background signal. Next, we detected a fungus, Candida albicans, with the described liquid phase immunoassay. Good relationship was obtained between the detected signal and the number of fungi. The minimum detectable number of fungi was as small as 30.
- 2007-11-15
著者
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Enpuku Keiji
Research Institute Of Superconductor Science And Systems Kyushu University
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TANAKA Tsuyoshi
Research Center for Integrated Systems, Hiroshima University
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Hamasaki Naotaka
Faculty Of Pharmaceutical Sciences Nagasaki International University
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Enomoto Naoya
Department Of Applied Chemistry Faculty Of Engineering Kyushu University
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Yoshinaga Kohji
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Kuma Hiroyuki
Faculty Of Pharmaceutical Sciences Nagasaki International Univ
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Dang Feng
Department Of Applied Chemistry Faculty Of Engineering Kyushu University
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Hojo Junichi
Department Chemistry & Biochemistry, Graduate School of Engineering, Kyushu University
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Kuma Hiroyuki
Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki 859-3298, Japan
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Matsuda Takashi
Research Institute of Superconductor Science and Systems, Kyushu University, Fukuoka 819-0395, Japan
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Ludwig Frank
Institute of Electrical Measurement and Fundamental Electrical Engineering, TU Braunschweig, Hans-Sommer-Str. 66, D-38106 Braunschweig, Germany
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Ghaffari Fatemeh
Institute of Electrical Measurement and Fundamental Electrical Engineering, TU Braunschweig, Hans-Sommer-Str. 66, D-38106 Braunschweig, Germany
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Heim Erik
Institute of Electrical Measurement and Fundamental Electrical Engineering, TU Braunschweig, Hans-Sommer-Str. 66, D-38106 Braunschweig, Germany
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Schilling Meinhard
Institute of Electrical Measurement and Fundamental Electrical Engineering, TU Braunschweig, Hans-Sommer-Str. 66, D-38106 Braunschweig, Germany
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Hojo Junichi
Department of Applied Chemistry, Kyushu University, Fukuoka 819-0395, Japan
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Enomoto Naoya
Department of Applied Chemistry, Kyushu University, Fukuoka 819-0395, Japan
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Hamasaki Naotaka
Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki 859-3298, Japan
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Tanaka Tsuyoshi
Research Institute of Superconductor Science and Systems, Kyushu University, Fukuoka 819-0395, Japan
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