Trapping and Collection of Lymphocytes Using Microspot Array Chip and Magnetic Beads
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概要
- 論文の詳細を見る
A microspot array chip, which has microspots of a magnetic thin film patterned on a glass substrate, was fabricated for trapping individual cells and for measuring their cellular response. The chip was easily fabricated by conventional semiconductor fabrication techniques on a mass production level as a disposable medical device. When a solution of lymphocyte-bound-magnetic beads was poured into the magnetized chip, each lymphocyte was trapped on each microspot of the magnetic thin film. The trapped cells were easily recovered from the chip using a micromanipulator. The micro-spot array chip can be utilized for arraying live cells and for measuring the response of each cell. The chip will be useful for preparing on array of different kinds of cells and for analyzing cellular response at the single cell level. The chip will be particularly useful for detecting antigen-specific B-lymphocytes and antigen-specific antibody complementary deoxyribonucleic acid (cDNA).
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2006-04-15
著者
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Kishi Hiroyuki
Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Japan
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Tanino Katsumi
Toyama Industrial Technology Center
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Fujiki Satoshi
Toyama Industrial Technology Center
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Muraguchi Atsushi
Department Of Immunology Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Tokimitsu Yoshiharu
Department Of Internal Medicine (3) University Of Toyama
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Obata Tsutomu
Toyama Industrial Technology Center, 150 Futagami, Takaoka, Toyama 933-0981, Japan
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Hashioka Shingi
Toyama New Industry Organization, 527 Takada, Toyama 930-0866, Japan
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Nakazato Hiroyoshi
Toyama New Industry Organization, 527 Takada, Toyama 930-0866, Japan
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Kishi Hiroyuki
Department of Immunology, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan
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Tokimitsu Yoshiharu
Department of Immunology, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan
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Kishi Hiroyuki
Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine
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