Deoxyribonucleic Acid Interaction with Silanized Surfaces
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概要
- 論文の詳細を見る
We demonstrate here the observation of the interaction between DNA and silanized cover glass surfaces using a fluorescence microscope and an atomic force microscope (AFM). Two-component surface modification with one alkylsilane mixed with another silane derivative containing amino end groups enhanced the adsorption of negatively charged DNA molecules due to the electrostatic interaction with positive charges of protonated amino end groups. We investigated the electrostatic interaction of the DNA molecules with the mixed silanized surfaces as a function of the concentration of the amino end groups. In addition, interaction on molecular scales was demonstrated by a newly developed double combing method.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2004-09-15
著者
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Fujihira Masamichi
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Kudo Hidetoshi
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Kwak Kwang
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Yoda Sawako
Department Of Biomolecular Engineering Tokyo Institute Of Technology
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Han Seung
Department Of Biological Sciences Korea Advanced Institute Of Science And Technology
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Kwak Kwang
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta Midori-ku, Yokohama 226-8501, Japan
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Han Seung
Department of Biological Science, Inje University
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