On-Chip Multichannel Action Potential Recording System for Electrical Measurement of Single Neurites of Neuronal Network

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概要

• 論文の詳細を見る

• We have developed a multielectrode array recording system for single-neurite-firing measurement using an artificially constructed neuronal network on a chip, which has a 10 μm diameter array with electrodes spaced at 50 μm, for noninvasive 64-channel 100 kHz multirecording and the stimulation of a plurality of neurites extending from a single neuron. To improve the signal/noise ratio, the ground plane was set on the multi-electrode-array plane and platinum black was set on each of the 10 μm electrodes. Using this system, we performed a multisite recording of neurites of a single neuron of a rat hippocampal network in cases of both spontaneous firing and evoked responses to electrical stimulations, and estimated the velocity of action potential propagation among neurites of a single neuron from six recording sites. This demonstrated the potential use of our low-noise chip and our high-speed measurement system for the analysis of neuronal network activities at the single-neuron level.

• Japan Society of Applied Physicsの論文
• 2007-11-25

著者

• Yasuda Kenji

  Department Of Biomedical Information Division Of Biosystems Institute Of Biomaterials And Bioenginee

• Suzuki Ikurou

  Department Of Biomedical Information Institute Of Biomaterials And Bioengineering Tokyo Medical And

• Hattori Akihiro

  Department Of Bio-analytical Chemistry Graduate School Of Pharmaceutical Sciences The University Of

• Yasuda Kenji

  Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan

• Hattori Akihiro

  Department of Biomedical Information, Division of Biosystems, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan

• Suzuki Ikurou

  Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan

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