On-Chip Single-Cell Observation Assay for Propagation Dynamics of Yeast Sup35 Prionlike Proteins

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概要

• 論文の詳細を見る

• The cytoplasmically inherited genetic determinant [PSI+] of the yeast Saccharomyces cerevisiae is presumed to be a manifestation of the prionlike properties of the chromosome-encoded Sup35 protein. Here, we show the relationship between the cell growth and the inheritance of Sup35p-GFP aggregation using on-chip single-cell observation assay. When Sup35 was expressed by induction, an aggregation was started after soluble Sup35-GFP in the cytoplasm reached to the critical concentration. Once the aggregation was generated, the concentration of free Sup35-GFP remained constant at a critical value. After stopping the Sup35-GFP induction by changing the cultivation buffer, the concentration of soluble Sup35-GFP remained constant, whereas the size of aggregation decreased. This result indicates that the aggregation of Sup35-GFP is a reversible growth/shrinking phenomenon with the same velocity constant as that for a forward/reverse reaction.

• 2004-11-01

著者

• Taguchi Hideki

  Department Of Biomolecular Engineering Tokyo Institute Of Technology

• Yasuda Kenji

  Department Of Biomedical Information Division Of Biosystems Institute Of Biomaterials And Bioenginee

• Yoshida Masasuke

  Chemical Research Laboratory Tokyo Institute Of Technology

• Taguchi Hideki

  Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan

• Yoshida Masasuke

  Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan

• Ayano Satoru

  Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan

• Noma Shigeko

  Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan

• Yasuda Kenji

  Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan

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