Transfer Agent of Immunity:II. Conversion of Nonimmune Spleen Cells into Antibody- Forming Cells by Transfer Agent in Ribonucleic Acid Fraction of Immunized Mice
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概要
- 論文の詳細を見る
Using erythrocytes as antigen particles, number of antibody-forming cells was enumer-ated by immunocytoadehesion technique, in which formation of rosette was shown to be inhibited by anti-mouse immunoglobulin sera. This number increased in vitro after treatment of spleen cells of mice for 60min with RNA fraction extracted from spleen of mice immunized with erythrocytes used in the enumeration, and incubation of cells for 12 hr at 37C. Response of cells treated with immune RNA fraction was immunologically specific and was inhibited by puromycin or cycloheximide. The activity of immune RNA capable of converting nonimmune cells to antibody-forming cells was shown to be sensi-tive to ribonucleases but resistant to deoxyribonuclease and proteolytic enzyme.
- 財団法人 学会誌刊行センターの論文
著者
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Mikami Hirokazu
Department Of Biology Faculty Of Social And Information Studies Gunma University
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Kawakami Masaya
Department Of Molecular Biology Kitasato University School Of Medicine
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Kitamura Kouichi
Department Of Cardiovascular Medicine Nephrology And Neurology Faculty Of Medicine And Dialysis Unit
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KITAMURA Kouichi
Department of Microbiology, School of Medicine, Gunma University
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MITSUHASHI Susumu
Department of Microbiologr, School of Medicine, Gunma University
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