Generation of Anti-DNA Specific Antibody Forming Cells from the Cultures of Human Peripheral Lymphocytes

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概要

• 論文の詳細を見る

• In vitro immune responses to calf thymic and human placental DNA associated with the maturation of antigen specific antibody-forming plasma cells from human peripheral lymphocyte cultures were studied in relation to T-cell homogenates derived from systemic lupus erythematosus (SLE) patients with high titers of anti-DNA antibodies in sera. T-cell homogenates of SLE patients converted normal B-cells to anti-DNA specific antibody-forming plasma cells, which were identified by the flocculation method using latex particles coated with antigen DNA. Anti-DNA specific antibody titers in culture medium were measured by radioimmunoassay, showing high antibody-titers in the cultures stimulated by SLE T-cell homogenates. Although anti-DNA specific antibody-forming cells were occasionally enumerated in normal B-cell cultures stimulated with normal T-cell homogenates, anti-DNA titers were generally lower than those stimulated by SLE T-cell homogenates. "Anti-DNA index" calculated by dividing anti-DNA antibody titers in culture media by the number of anti-DNA specific plasma cells, demonstrated active production of anti-DNA antibodies per 10,000 plasma cells stimulated by SLE T-cell homogenates. Normal T-cell homogenates sometimes suppressed the formation of anti-DNA specific antibodyforming plasma cells from normal and occasionally from SLE B-cell cultures. Some SLE B-cell cultures were insensitive to normal T-cell homogenates, and produced high amounts of anti-DNA antibodies. These results indicate that each SLE patient has B-cells in distinct stages of differentiation. In other words, B-cells were sometimes primed with DNA and sometimes stayed in innate immunoblasts, when they were isolated. Suppressive effect of normal T-cell homogenates seem to be effective only on immunoblasts just communicating with other types of cells. Once B-cells begin to differentiate toward specific antibody-forming cells, the suppressive effect of normal T-cell homogenates seems to be powerless in prohibiting B-cell differentiation. Regulation of anti-DNA specific antibody-forming plasma cells seems to be performed on a delicate balance between T-cell subsets and B-cell lineages.

• 北里大学の論文
• 1980-12-31

著者

• Yoshii Akio

  Department Of Internal Medicine Kitasato University School Of Medicine

• Kawakami Masaya

  Department of Molecular Biology, School of Medicine, Kitasato University.

• Kashiwazaki Sadao

  Department Of Internal Medicine Kitasato University School Of Medicine

• Kashiwazaki Sadao

  Department Of Clinical Internal Medicine Kitasato University School Of Medicine

• Nakamura Kunie

  Department Of Molecular Biology Laboratory Kitasato University School Of Medicine

• Kawakami Masaya

  Department Of Molecular Biology Kitasato University School Of Medicine

• Kawakami Masaya

  Department Of Molecular Biology Laboratory Kitasato University School Of Medicine

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