Identification of the Source of Francisella tularensis Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis
スポンサーリンク
概要
- 論文の詳細を見る
Tularemia is a zoonotic disease caused by Francisella tularensis. Most patients in Japan have reportedly acquired such infections through direct contact with infected Japanese hares. We recently encountered a patient who contracted tularemia after skinning and butchering a dead hare. Because the remains of the hare were available, we attempted to determine whether the patient actually contracted infection by handling the carcass. F. tularensis-specific sequences were successfully amplified by PCR from the patient specimens as well as from the remnants of discarded hare carcass. PCR amplification of the ISFtu2 and RD1 regions indicated infection by F. tularensis subsp. holarctica, which was considered as a prevalent strain in Japan. Furthermore, high-resolution multiple-locus variable-number tandem repeat analysis (MLVA) showed that the combination of repeat numbers in sequences from the patient and hare were indistinguishable, thus indicating that the patient had been infected with F. tularensis strain that had also infected the hare. These findings demonstrated that MLVA is a useful epidemiological investigational tool to identify possible sources of certain zoonotic diseases such as tularemia.
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committeeの論文
著者
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Hotta Akitoyo
Department Of Veterinary Microbiology Faculty Of Agriculture Gifu University
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Asano Shigeyuki
Department Of Pathology Iwaki Kyoritsu General Hospital
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Shinya Fumiaki
Department Of Surgery Iwaki-kyoritsu Hospital
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Uda Akihiko
Department Of Veterinary Science National Institute Of Infectious Diseases
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Tanabayashi Kiyoshi
Department Of Veterinary Science National Institute Of Infectious Diseases
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Morikawa Shigeru
Department Of Bacteriology And Department Of Pathology Chest Disease Research Institute Kyoto Univer
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Yamamoto Yoshie
Department Of Veterinary Science
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Fujita Osamu
Department of Bioresources Science and Technology, Graduate School of Biosphere Science, Hiroshima University
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Hotta Akitoyo
Department of Veterinary Science, National Institute of Infectious Diseases
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YAMAMOTO Yoshie
Department of Veterinary Science, National Institute of Infectious Diseases
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Morikawa Shigeru
Department of Veterinary Science, National Institute of Infectious Diseases
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Fujita Hiromi
Fujita Health University
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Yamada Akio
Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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