Studies on the Distribution and Immunohistochemical Localization of Xanthine Oxidase in Bovine Tissues

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概要

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• Distribution of xanthine oxidase activity in bovine tissues was investigated by biochemical assay and cellular localization of the enzyme was studied using a fluorescent antibody technique. XOD activity was detected in the homogenate of liver, spleen, duodenum, lung and kidney. Liver and spleen had at least five times higher activity. Fractionation of liver homogenate revealed the presence of enzyme activity only in the high speed supernatant fraction. Immunochemical properties of XODs in the soluble fractions from each organ homogenate were studied using antibody to milk XOD. The enzyme in these organs and that from milk revealed complete immunological identity. Immunohistochemically, in cryostat sections fixed with a strong fixative such as Carnoy's solution etc. the enzyme was localized as follows : Liver; in the cytoplasm of hepatic cells. Duodenum; in the apical portion of Brunner's gland epithelium. Kidney; in some of the epithelium of the proximal tubuli. Mammary gland; in the apex of alveolar epithelium and in the margin of alveolar spaces. Although the choice of the fixatives for immunohistochemical studies was empirical, possible reasons for lack of effect with fixatives employed in the present study are discussed.

• 島根大学の論文

著者

• HARADA Takayuki

  Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience

• MORIKAWA Shigeru

  Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, U

• Morikawa S

  Department Of Pathology First Unit Shimane Medical University

• Harada Takayuki

  Second Unit Shimane Medical University

• Harada Takayuki

  Department Of Pathology Shimane Medical University

• Harada Takayuki

  Department Of Pathology Shimane Medical University:orchard Park Laboratories Roswell Park Memorial I

• SUZUKI Yukio

  Department of Pathology, Shimane Medical University

• Harada Takayuki

  Department Of Molecular Neurobiology Tokyo Metropolitan Institute For Neuroscience

• BABA MITSUO

  Department of Pathology, Chest Disease Research Institute, Kyoto University

• YASUHIRA KIMIO

  Department of Pathology, Chest Disease Research Institute, Kyoto University

• Morikawa Shigeru

  Department Of Pathology Shimane Medical University

• Baba Mitsuo

  Department Of Pathology Chest Disease Research Institute Kyoto University

• Baba Mitsuo

  Department Of Bacteriology And Department Of Pathology Chest Disease Research Institute Kyoto Univer

• Suzuki Yukio

  Department Of Pathology And Internal Medicine Shimane Medical University

• Yasuhira Kimio

  Department Of Pathology Chest Disease Research Institute Kyoto University

• Harada Takayuki

  Dept. Of Pathology Shimane Medical Univ.

• Harada Takayuki

  Dept Of Pathology Shimane Medical University

• Morikawa Shigeru

  Department Of Bacteriology And Department Of Pathology Chest Disease Research Institute Kyoto Univer

• Suzuki Yukio

  Department Of Electrical Engineering School Of Science And Engineering Waseda University

• Suzuki Yukio

  Department of Chemistry, Faculty of Science, Hokkaido University

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