Immunohistochemical Localization of Lactate Dehydrogenase Subunits in Porcine Liver; Effect of Fixatives on Detection of Specific Fluorescence
スポンサーリンク
概要
- 論文の詳細を見る
Immunohistochemical localization of lactate dehydrogenase subunits was studied using a fluorescent antibody technique in porcine liver. Because of the difficulty in demonstrating specific fluorescence of LDH-M in the liver tissue, many fixatives were tested both in frozen and paraffin sections to obtain consistent staining results. Among the fixatives examined, ether-ethanol-acetone mixture in frozen sections gave the best result in demon-strating both subunits in the liver tissue by immunofluorescence. Paraffin sections failed to preserve LDH-M specific fluorescence in this study. Specific fluorescence for LDH-H was observed in the hepatic cell membrane facing the space of Disse, in the lining cells of sinusoids and in the cyto plasm of Kupffer cells. LDH-M specific fluorescence was observed as fine granules in the cytoplasm of hepatic cells.
- 島根大学の論文
著者
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HARADA Takayuki
Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience
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MORIKAWA Shigeru
Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, U
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Morikawa S
Department Of Pathology First Unit Shimane Medical University
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Harada Takayuki
Second Unit Shimane Medical University
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Harada Takayuki
Department Of Pathology Shimane Medical University
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Harada Takayuki
Department Of Pathology Shimane Medical University:orchard Park Laboratories Roswell Park Memorial I
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SUZUKI Yukio
Department of Pathology, Shimane Medical University
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Harada Takayuki
Department Of Molecular Neurobiology Tokyo Metropolitan Institute For Neuroscience
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ITO Motohiko
Department of Thoracic Surgery
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FUKUDA Haruo
Department of Thoracic Surgery
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Morikawa Shigeru
Department Of Pathology First Unit Shimane Medical University
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Morikawa Shigeru
Department Of Pathology Shimane Medical University
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Fukuda Haruo
Department Of Thoracic Surgery Chest Disease Research Institute Kyoto University
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Suzuki Yukio
Department Of Pathology And Internal Medicine Shimane Medical University
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Harada Takayuki
Dept. Of Pathology Shimane Medical Univ.
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Harada Takayuki
Dept Of Pathology Shimane Medical University
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Morikawa Shigeru
Department Of Bacteriology And Department Of Pathology Chest Disease Research Institute Kyoto Univer
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Suzuki Yukio
Department Of Electrical Engineering School Of Science And Engineering Waseda University
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Suzuki Yukio
Department of Chemistry, Faculty of Science, Hokkaido University
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